Gut microbiome of helminth-infected indigenous Malaysians is context dependent

Microbiome

Research

Angelina Angelova¹  Poorani Subramanian¹  Kek Heng Chua²  Mian Zi Tee²  Kee Seong Ng³  Joseph Devlin⁴  Ze Chen⁴  Alice V. Easton⁴  Shushan Sargsian⁵  Ken Cadwell⁶  Christopher Chiong Meng Boey⁷  Yi Xian Er⁸  Nan Jiun Yap⁸  Romano Ngui⁸  Yvonne Ai Lian Lim⁸  Daniel P. Beiting⁹  Ii Li Lee^1,10  Soo Ching Lee^1,11  P’ng Loke^1,11  Oyebola Oyesola^1,11 

[1] Bioinformatics and Computational Biosciences Branch, Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA;Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;Department of Gastroenterology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;Department of Microbiology, New York University Grossman School of Medicine, New York, NY, USA;Department of Microbiology, New York University Grossman School of Medicine, New York, NY, USA;Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY, USA;Department of Microbiology, New York University Grossman School of Medicine, New York, NY, USA;Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY, USA;Division of Gastroenterology, Department of Medicine, New York University Langone Health, New York, NY, USA;Department of Paediatrics, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA;Kulliyyah of Medicine and Health Sciences, University Islam Antarabangsa Sultan Abdul Halim Mu’adzam Shah, 09300, Kuala Ketil, Kedah, Malaysia;Type 2 Immunity Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD, USA;

关键词: Helminth;    Microbiome;    Metagenomic sequencing;    Indigenous population;    Albendazole;   

DOI  :  10.1186/s40168-022-01385-x

 received in 2022-01-21, accepted in 2022-10-04,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundWhile microbiomes in industrialized societies are well characterized, indigenous populations with traditional lifestyles have microbiomes that are more akin to those of ancient humans. However, metagenomic data in these populations remains scarce, and the association with soil-transmitted helminth infection status is unclear. Here, we sequenced 650 metagenomes of indigenous Malaysians from five villages with different prevalence of helminth infections.ResultsIndividuals from villages with higher prevalences of helminth infections have more unmapped reads and greater microbial diversity. Microbial community diversity and composition were most strongly associated with different villages and the effects of helminth infection status on the microbiome varies by village. Longitudinal changes in the microbiome in response to albendazole anthelmintic treatment were observed in both helminth infected and uninfected individuals. Inference of bacterial population replication rates from origin of replication analysis identified specific replicating taxa associated with helminth infection.ConclusionsOur results indicate that helminth effects on the microbiota were highly dependent on context, and effects of albendazole on the microbiota can be confounding for the interpretation of deworming studies. Furthermore, a substantial quantity of the microbiome remains unannotated, and this large dataset from an indigenous population associated with helminth infections is a valuable resource for future studies.AEGeXaED_X6wKACk1ZMX_FVideo Abstract

【 授权许可】

CC BY   
© The Author(s) 2022

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