Cuscuta epithymum Murr. crude extract pre-conditioning protects C6 cells from L-glutamate-induced neurotoxicity

BMC Complementary Medicine and Therapies

Research

Mina Soufi Zomorrod¹  Faraz Mojab²  Mehrdad Faizi³  Rasoul Ghasemi⁴  Masoumeh Pourhadi⁵  Hakimeh Zali⁶  Zahra Niknam⁷  Vahid Niazi⁸ 

[1] Department of Hematology and Cell Therapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Physiology and Neurophysiology research center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Neurophysiology Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Science, Urmia, Iran;Proteomics Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran;Stem Cell Research Center, Golestan University of Medical Science, Gorgan, Iran;Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Gorgan, Iran;

关键词: Cuscuta epithymum;    Crude extract;    Fractions;    Phenolic content;    Neuroprotective;    MDA;    SOD;    ROS;   

DOI  :  10.1186/s12906-022-03816-6

 received in 2022-08-10, accepted in 2022-12-02,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundCuscuta epithymum Murr. (C. epithymum), as an herbal medicine, has played an anti-cancerous role in various studies; however, its possible neuroprotective effects have been neglected. Here, we aimed to investigate the protective effects of C. epithymum seeds crude extract and different fractions on rat glioblastoma cells (C6) in L-glutamate oxidative condition.MethodsInitially, the total phenolic content of C. epithymum crude extract and the fractions (all produced by maceration method) was determined. Subsequently, C6 cells were pre-treated with the various concentrations of crude extract and fractions 24 h before L-glutamate exposure. Likewise, C6 cells were treated with the same concentrations of crude extract and fractions 24 h after exposure to L-glutamate. The cell viability and morphology were compared in crude extract and fractions groups, then superoxide dismutase (SODs) activity, reactive oxygen species (ROS), and malondialdehyde (MDA) levels were measured. The flow cytometry test was used to study C. epithymum crude extract's effects on the cell cycle and also to quantify the apoptosis, necrosis, and live cells population in different groups.ResultsC. epithymum crude extract and fractions (hexanoic, dichloromethanolic, and methanolic) had concentration-dependent cytotoxicity (IC50:126.47, 2101.96, 140.97, and 218.96 µg/ml, respectively). The crude extract and methanolic fraction contained phenolic compounds (55.99 ± 2.795 and 50.80 ± 2.969 mg gallic acid/g extract), while in hexanoic and dichloromethanolic fractions, the phenolic content was undetectable. In the cell viability assay, in comparison to fractions, the crude extract showed a more protective effect against glutamate-induced oxidative condition (P < 0.0001). The crude extract increased the SODs activity (P < 0.001) and decreased MDA and ROS levels (P < 0.0001) in comparison to the glutamate group. The crude extract significantly increased the population of cells in G1 (from 63.04 to 76.29) and decreased the percentage of cells in G2 (from 11.56 to 6.7) and S phase (from 25.4 to 17.01). In addition, it decreased the apoptotic and necrotic cell populations (from 34 to 17.1) and also increased the percentage of live cells (from 66.8 to 83.4 percent) in the flow cytometry test.ConclusionC. epithymum crude extract plays a neuroprotective role by activating the defense mechanisms in cell against the oxidative condition.

【 授权许可】

CC BY   
© The Author(s) 2022

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