CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant

Nano-Micro Letters

Communication

Omar A. Al-Hartomy¹  S. Wageh¹  Xiaopeng Ma²  Chaozhou Li³  Taojian Fan³  Han Zhang³  Chenshuo Wu³  Yuxuan Yuan³  Luxiao Chai³  Yuxuan Tang⁴  Zhi Chen⁴  Yabin Hao⁵  Jingfeng Li⁶  Abdullah G. Al-Sehemi⁷  Yaqing He⁸  Jiangtian Yu⁹  Zhongjian Xie^1,10  Zhiguang Luo^1,11 

[1] Department of Physics, Faculty of Science, King Abdulaziz University, 21589, Jeddah, Saudi Arabia;Department of Respiratory, Shenzhen Children’s Hospital, 518038, Shenzhen, People’s Republic of China;International Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen University, 518060, Shenzhen, People’s Republic of China;International Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen University, 518060, Shenzhen, People’s Republic of China;Hospital of Guangzhou Medical University, Qingyuan city People’s Hospital, 511518, Qingyuan, People’s Republic of China;International Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen University, 518060, Shenzhen, People’s Republic of China;Shenzhen Han’s Tech Limited Company, 518000, Shenzhen, People’s Republic of China;International Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen University, 518060, Shenzhen, People’s Republic of China;Shenzhen International Institute for Biomedical Research, 518116, Shenzhen, People’s Republic of China;Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, 61413, Abha, Saudi Arabia;Department of Chemistry, College of Science, King Khalid University, P.O. Box 9004, 61413, Abha, Saudi Arabia;Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, People’s Republic of China;Shenzhen International Institute for Biomedical Research, 518116, Shenzhen, People’s Republic of China;Shenzhen International Institute for Biomedical Research, 518116, Shenzhen, People’s Republic of China;Institute of Pediatrics, Shenzhen Children’s Hospital, 518038, Shenzhen, People’s Republic of China;Zhongmin (Shenzhen) Intelligent Ecology Co., Ltd, 518055, Shenzhen, People’s Republic of China;

关键词: SARS-COV-2 variant;    Methodology of electrochemical CRISPR sensing (MOECS);    Gold nanoparticles (AuNPs);    Point-of-care testing (POCT);   

DOI  :  10.1007/s40820-022-00888-4

 received in 2022-06-03, accepted in 2022-06-17,  发布年份 2022

来源: Springer

【 摘 要 】

tsA robust technique-methodology of electrochemical CRISPR sensing is first proposed for the rapid, highly sensitive and specific detection of SARS-CoV-2 variant without any nucleic-acid-amplification assays.Using the DNA template identical to SARS-CoV-2 Delta spike gene sequence as model, our biosensor exhibited excellent analytical detection limit (50 fM) and high linearity (R2 = 0.987) without any amplification assay.Specific crRNA was designed to match the mutation site on nucleic acid sequence of the SARS-CoV-2 Delta variant, presenting programmability, universality, and scalability for diagnosis of other emerging SARS-CoV-2 variants.

【 授权许可】

CC BY   
© The Author(s) 2022

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