A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe

Journal of Nanobiotechnology

Yang Zhang¹  Qinghai Chen²  Fengxin Xie²  Changjing Yuan²  Ming Chen²  Ke Xia²  Zhi Li²  Huiyan Tian²  Weiling Fu²  Mengya Li²  Yu Liu² 

[1] Department of Laboratory Medicine, Chongqing University Cancer Hospital, Chongqing, China;Department of Laboratory Medicine, First Affiliated Hospital, Third Military Medical University (Army Medical University), Chongqing, China;

关键词: Allosteric spherical nanoprobe;    Dual-hairpin;    MiRNA-150;    Förster resonance energy transfer (FRET);    Point-of-care testing (POCT);   

DOI  :  10.1186/s12951-020-00717-z

来源: Springer

【 摘 要 】

MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer (NSCLC), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC. Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield “signal on” fluorescence. In the manner of multipoint fluorescence detection, the target-bound allosteric spherical nanoprobe could provide high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method can distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

【 授权许可】

CC BY