Detection and sequence analysis of Canine morbillivirus in multiple species of the Mustelidae family

BMC Veterinary Research

Research

József Lanszki¹  Gábor Csorba²  Tamás Cserkész²  András István Csathó³  Tamás Görföl⁴  Ferenc Jakab⁵  Zsófia Lanszki⁵  Gábor Endre Tóth⁵  Gábor Kemenesi⁵ 

[1] Balaton Limnological Research Institute, 8237, Tihany, Hungary;Hungarian University of Agriculture and Life Sciences, 7400, Kaposvár, Hungary;Department of Zoology, Hungarian Natural History Museum, 1088, Budapest, Hungary;Independent Researcher, 5830, Battonya, Hungary;National Laboratory of Virology, University of Pécs, 7624, Pécs, Hungary;National Laboratory of Virology, University of Pécs, 7624, Pécs, Hungary;Institute of Biology, Faculty of Sciences, University of Pécs, 7624, Pécs, Hungary;

关键词: Carnivora;    Mustelids;    NGS;    MinION;    Third generation sequencing;    Protected species;    Wildlife disease;    Disease ecology;    Tailing;    Canine distemper virus;   

DOI  :  10.1186/s12917-022-03551-7

 received in 2022-09-22, accepted in 2022-12-13,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundCanine morbillivirus (canine distemper virus, CDV) is a member of the Paramyxoviridae family. Canine distemper is a serious viral disease that affects many mammalian species, including members of the Mustelidae family. These animals have an elusive nature, which makes related virological studies extremely challenging. There is a significant knowledge gap about the evolution of their viruses and about the possible effects of these viruses to the population dynamics of the host animals. Spleen and lung tissue samples of 170 road-killed mustelids belonging to six species were collected between 1997 and 2022 throughout Hungary and tested for CDV with real-time RT-PCR.ResultsThree species were positive for viral RNA, 2 out of 64 Steppe polecats (Mustela eversmanii), 1 out of 36 European polecats (Mustela putorius) and 2 out of 36 stone martens (Martes foina); all 18 pine martens (Martes martes), 10 least weasels (Mustela nivalis) and 6 stoats (Mustela erminea) tested negative. The complete CDV genome was sequenced in five samples using pan-genotype CDV-specific, amplicon-based Nanopore sequencing. Based on the phylogenetic analysis, all five viral sequences were grouped to the Europe/South America 1 lineage and the distribution of one sequence among trees indicated recombination of the Hemagglutinin gene. We verified the recombination with SimPlot analysis.ConclusionsThis paper provides the first CDV genome sequences from Steppe polecats and additional complete genomes from European polecats and stone martens. The infected specimens of various species originated from distinct parts of the country over a long time, indicating a wide circulation of CDV among mustelids throughout Hungary. Considering the high virulence of CDV and the presence of the virus in these animals, we highlight the importance of conservation efforts for wild mustelids. In addition, we emphasize the importance of full genomic data acquisition and analysis to better understand the evolution of the virus. Since CDV is prone to recombination, specific genomic segment analyses may provide less representative evolutionary traits than using complete genome sequences.

【 授权许可】

CC BY   
© The Author(s) 2022

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Fig. 3