Green tea catechin-grafted silk fibroin hydrogels with reactive oxygen species scavenging activity for wound healing applications

Biomaterials Research

Research Article

Young-Gwang Ko¹  Gyeongwoo Lee¹  Oh Hyeong Kwon¹  Oh Kyoung Kwon²  Ki Hyun Bae³  Motoichi Kurisawa³ 

[1] Department of Polymer Science and Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, Korea;Gastrointestinal surgery, Kyungpook National University Chilgok Hospital, Daegu 41404, Korea;Department of Surgery, Kyungpook National University School of Medicine, Daegu 41944, Korea;Institute of Bioengineering and Bioimaging, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore;

关键词: Silk fibroin;    EGCG;    Hydrogel;    Reactive oxygen species;    Wound healing;   

DOI  :  10.1186/s40824-022-00304-3

 received in 2022-07-14, accepted in 2022-10-05,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundOverproduction of reactive oxygen species (ROS) is known to delay wound healing by causing oxidative tissue damage and inflammation. The green tea catechin, (–)-Epigallocatechin-3-O-gallate (EGCG), has drawn a great deal of interest due to its strong ROS scavenging and anti-inflammatory activities. In this study, we developed EGCG-grafted silk fibroin hydrogels as a potential wound dressing material.MethodsThe introduction of EGCG to water-soluble silk fibroin (SF-WS) was accomplished by the nucleophilic addition reaction between lysine residues in silk proteins and EGCG quinone at mild basic pH. The resulting SF-EGCG conjugate was co-crosslinked with tyramine-substituted SF (SF-T) via horseradish peroxidase (HRP)/H2O2 mediated enzymatic reaction to form SF-T/SF-EGCG hydrogels with series of composition ratios.ResultsInterestingly, SF-T70/SF-EGCG30 hydrogels exhibited rapid in situ gelation (< 30 s), similar storage modulus to human skin (≈ 1000 Pa) and superior wound healing performance over SF-T hydrogels and a commercial DuoDERM® gel dressings in a rat model of full thickness skin defect.ConclusionThis study will provide useful insights into a rational design of ROS scavenging biomaterials for wound healing applications.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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