Genes & Nutrition | |
Comparison of apical and basolateral Cu treatment for iron-related gene regulation during deferoxamine induced iron deficiency | |
Research | |
Sukru Gulec1  Ezgi Evcan1  | |
[1] İzmir Institute of Technology, Faculty of Engineering, Department of Food Engineering, Molecular Nutrition and Human Physiology Laboratory, Urla, 35430, İzmir, Turkey; | |
关键词: Iron deficiency; Anemia; Copper; Iron; Intestine; Caco-2; Apical; Basolateral; | |
DOI : 10.1186/s12263-022-00717-8 | |
received in 2021-02-22, accepted in 2022-11-10, 发布年份 2022 | |
来源: Springer | |
【 摘 要 】
BackgroundIntestinal copper transporter (Atp7a) mutant-brindled mice with systemic Cu deficiency had elevated Cu levels in enterocyte cells without any perturbation of iron-regulating genes, suggesting that blood Cu level might be important for intestinal iron homeostasis during iron deficiency (ID). We hypothesized that the blood Cu level and polarization (apical and basolateral) of enterocyte cells might be important regulators for the compensatory response on the regulation of genes in enterocyte cells during iron deficiency.MethodsWe grew Caco-2 cells on a bicameral cell culture plate to mimic the human intestine system and on a regular tissue culture plate. Iron deficiency was induced by deferoxamine (DFO). The cells were treated with Cu and Cu with Fe following mRNA expressions of DMT1, FPN, TFR, and ANKRD37 were analyzed.ResultsOur main finding was that basolateral treatment of Cu significantly reduced mRNA expressions of iron-regulated genes, including DMT1, FPN, TFR, and ANKRD37, compared to DFO-treated and DFO with apical Cu-treated groups in both bicameral and regular tissue culture plates.ConclusionsCu level in the basolateral side of Caco-2 cells significantly influenced the intracellular gene regulation in DFO-induced iron-deficient condition, and polarization of the cells might be important factor gene regulation in enterocyte cells.
【 授权许可】
CC BY
© The Author(s) 2022
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202305067182584ZK.pdf | 1196KB | download | |
12982_2022_119_Article_IEq22.gif | 1KB | Image | download |
12982_2022_119_Article_IEq23.gif | 1KB | Image | download |
12982_2022_119_Article_IEq26.gif | 1KB | Image | download |
【 图 表 】
12982_2022_119_Article_IEq26.gif
12982_2022_119_Article_IEq23.gif
12982_2022_119_Article_IEq22.gif
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]