Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis

Biomaterials Research

Research Article

Yuan Yuan¹  Changsheng Liu¹  Kai Chen¹  Qiyu Luo²  Cunyi Fan²  Tengfei Lou² 

[1] Key Laboratory for Ultrafine Materials of Ministry of Education, and School of Materials Science and Engineering, East China University of Science and Technology, 200237, Shanghai, People’s Republic of China;Frontiers Science Center for Materiobiology and Dynamic Chemistry, and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, 200237, Shanghai, People’s Republic of China;Orthopaedic Department, Shanghai Sixth People’s Hospital, 200233, Shanghai, People’s Republic of China;

关键词: In situ precipitated CaP nanoparticles;    Nanocomposite hydrogels;    Bone adhesion;    Stretchability;    Dynamic distraction osteogenesis;    Mechanical stimulation;   

DOI  :  10.1186/s40824-022-00330-1

 received in 2022-08-16, accepted in 2022-11-27,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundDistraction osteogenesis (DO) is an efficacious but lengthy procedure to reconstruct segmental bone defects under the principle of tension-stress, during which the periosteum-mediated mechanical stimulation plays a pivotal role. Inspired by the dynamic process of DO and the mechanical stimulation of periosteum, a new design of bionic periosteum was developed to simulate the mechanical transduction of natural periosteum for the application in DO procedure.MethodsIn this study, an injectable organic-inorganic hybrid hydrogel was developed based on a novel combination of the PEGylated poly (glycerol sebacate) (PEGS) polymer network and in situ formed CaP nanoparticles (ICPNs). Rat bone marrow mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured and tested in vitro to evaluate biocompatibility, cell adhesion, proliferation, and pro-osteogenic and pro-angiogenic activity. In vivo experiments were conducted in the rat tibial model of distraction osteogenesis.ResultsThe developed nanocomposite hydrogels exhibited excellent injectability, robust bone adhesion, superior stretchability, and enhanced osteogenic activity. The results of in vitro and in vivo studies showed that PEGS/ICPN hydrogels could promote new bone formation and mineralization during the dynamic distraction process through the synergistic effects of angiogenesis and osteogenesis.ConclusionsThis periosteum-inspired nanocomposite hydrogel represents a mechanobiology approach for effectively restoring large bone defects through the dynamic DO process.

【 授权许可】

CC BY   
© The Author(s) 2022

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