The hsa_circ_0039857/miR-338-3p/RAB32 axis promotes the malignant progression of colorectal cancer

BMC Gastroenterology

Research

Xiaoyan He¹  Zhirong Yang¹  Siying Cheng¹  Xianwei Wang¹  Pei Xu¹  Ning Wu¹  Shuming Jiang¹  Lina Tang¹ 

[1] Department of Pathology, People’s Hospital of De Yang City, No. 173, Section 1 of North Taishan Road, Jingyang District, 618000, Deyang, Sichuan, China;

关键词: Colorectal cancer;    hsa_circ_0039857;    miR-338-3p;    RAB32;    Cancer progression;   

DOI  :  10.1186/s12876-022-02622-1

 received in 2022-05-19, accepted in 2022-12-15,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundColorectal cancer (CRC) is a prevalent malignancy of the gastrointestinal. Circular RNAs (circRNAs) act as important roles in CRC malignant progression. However, the role of circ_0039857 in CRC is still unclear. Therefore, this study aimed to explore the function and mechanism of hsa_circ_0039857 in the CRC.MethodsThe mRNA and protein expression were measured via RT-qPCR. RNase R assay and Actinomycin D were employed to evaluate the stability of circ_0039857. Functional experiments, such as proliferation and apoptosis, were applied to study the function of circ_0039857 in CRC cells. The underlying mechanisms of circ_0039857 were then analyzed by bioinformatics, dual-luciferase reporter gene assay, RNA pull-down and rescue experiments.ResultsWe revealed that circ_0039857 was significantly enhanced in CRC. Circ_0039857 was stabler than linear RNA in cells and valuable for the disease diagnosis. In addition, circ_0039857 knockdown inhibited proliferation and promoted apoptosis. Mechanistically, circ_0039857 positively regulated the expression of RAB32 via sponging miR-338-3p.ConclusionThis study demonstrated that circ_0039857 knockdown suppressed CRC malignant progression through miR-338-3p/RAB32 axis. Most importantly, this will help us to better understand the circRNA network in CRC, and may find potential biomarkers and targets for CRC clinical treatment.

【 授权许可】

CC BY   
© The Author(s) 2022

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