A base substitution in OsphyC disturbs its Interaction with OsphyB and affects flowering time and chlorophyll synthesis in rice

BMC Plant Biology

Research

Yuchun Rao¹  Xiaoli Lin²  Jianmin Bian²  Changlan Zhu²  Junru Fu²  Linjuan Ouyang²  Jie Xu²  Dahu Zhou²  Haohua He²  Chunlian Chen³  Guoxing Zou³  Jianhua Yin³  Yongping Huang³ 

[1] College of Chemistry and Life Sciences, Zhejiang Normal University, 321004, Jinhua, China;Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, 330045, Nanchang, China;National Engineering Laboratory of Rice (Nanchang), Rice Research Institute, Jiangxi Academy of Agricultural Sciences, 330200, Nanchang, China;

关键词: Phytochrome C;    Flowering time;    Chlorophyll synthesis;    Dimerization;    Rice;   

DOI  :  10.1186/s12870-022-04011-y

 received in 2022-08-24, accepted in 2022-12-19,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundPhytochromes are important photoreceptors in plants, and play essential roles in photomorphogenesis. The functions of PhyA and PhyB in plants have been fully analyzed, while those of PhyC in plant are not well understood.ResultsA rice mutant, late heading date 3 (lhd3), was characterized, and the gene LHD3 was identified with a map-based cloning strategy. LHD3 encodes phytochrome C in rice. Animo acid substitution in OsphyC disrupted its interaction with OsphyB or itself, restraining functional forms of homodimer or heterodimer formation. Compared with wild-type plants, the lhd3 mutant exhibited delayed flowering under both LD (long-day) and SD (short-day) conditions, and delayed flowering time was positively associated with the day length via the Ehd1 pathway. In addition, lhd3 showed a pale-green-leaf phenotype and a slower chlorophyll synthesis rate during the greening process. The transcription patterns of many key genes involved in photoperiod-mediated flowering and chlorophyll synthesis were altered in lhd3.ConclusionThe dimerization of OsPhyC is important for its functions in the regulation of chlorophyll synthesis and heading. Our findings will facilitate efforts to further elucidate the function and mechanism of OsphyC and during light signal transduction in rice.

【 授权许可】

CC BY   
© The Author(s) 2022

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