Anti-glycation, antiplatelet and antioxidant effects of different pomegranate parts

BMC Complementary Medicine and Therapies

Research

Amira Zarrouk¹  Raja Chaaba¹  Mohamed Hammami¹  Sonia Hammami¹  Zahra Amri¹  Jalel Gargouri²  Ikram Ben Amor² 

[1] Biochemistry Laboratory, LR12ES05 “Nutrition- Functional Foods and vascular Health”, Faculty of Medicine, University of Monastir, 5019, Monastir, Tunisia;Centre Régional de Transfusion Sanguine de Sfax, Route El-Ain Km 0.5, CP 3003, Sfax, Tunisia;

关键词: Pomegranate;    Antiplatelet activity;    Advanced glycation end-products;    Oxidative stress;    Lipid peroxidation;   

DOI  :  10.1186/s12906-022-03824-6

 received in 2022-08-22, accepted in 2022-12-16,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundPlatelet aggregation and advanced glycation end products (AGEs) and oxidative stress are known as key factors for the development of cardiovascular diseases and diabetic complications. In this context, fruit and vegetable consumption, good sources of antioxidant compounds have been largely reported as an effective way of preventing human against these diseases. The current study focuses on the evaluation of antioxidant, antiplatelet and anti-glycation activities of pomegranate (Punica granatum L.) flowers (PF), leaves (PL), peel (PP) juice (PJ) and seeds oil (PSO).MethodsAntioxidant activities was measured against ABTS radical and lipid peroxidation. Antiglycation activity was determined using the formation of AGE fluorescence intensity in the BSA/ribose system. Antiplatelet activity was measured in platelet rich plasma (PRP) against adenosine diphosphate (ADP), Collagen and arachidonic acid (AA).ResultsPF extract displayed the highest antioxidant activity against ABTS and lipid peroxidation with IC50 values of 0.7 mg/mL and 0.63 mg/mL respectively. For anti-glycation activity, PP, PF and PL inhibited moderately the pentosidine-like AGEs formation compared to positive controls with AGE-IC50 value of 0.4 mg/mL. PJ and PSO haven’t any anti-AGE effect. All the extracts selectively inhibited platelet aggregation caused by one, two or three inducers in dose dependent manner. PF was the most potent inhibitor caused by all three inducers, with inhibitory effects ranging from 35.6 to 66.6%. PP and PJ exhibited antiplatelet effect against both ADP and collagen and PL and PSO only against AA.ConclusionsThese results suggest that some pomegranate extracts exert potential in vitro anti-glycative and antiplatelet activities.

【 授权许可】

CC BY   
© The Author(s) 2022