Diallyl trisulfide modulated autophagy in isoproterenol induced acute myocardial infarction

Clinical Phytoscience

Original Contribution

Naglaa F. Khedr¹  Nahla E. El-Ashmawy¹  Ghada M. Al-Ashmawy¹  Mohamed N. Shaban² 

[1] Department of Biochemistry, Faculty of Pharmacy, Tanta University, Elgeesh Street, Medical compound, 31527, Al-Garbia, Egypt;El Shehydi hospital, 31818, Garbia, Egypt;

关键词: Autophagy;    Captopril;    DATS;    Isoproterenol;    Acute myocardial infarction;    PI3K/Akt pathway;   

DOI  :  10.1186/s40816-022-00351-2

 received in 2022-04-17, accepted in 2022-11-05,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundAcute myocardial infarction (AMI) is the most serious manifestation of coronary artery disease. The initial ischemia in AMI causes biochemical and metabolic alterations in cardiomyocytes.ObjectivesThe present study aimed to investigate the biomolecular mechanisms underlying cardioprotective effects of diallyl trisulfide (DATS) as well as captopril (CAP) in isoproterenol (ISO) induced AMI focusing on autophagy & PI3K/Akt signaling.MethodsSeventy male Albino rats were divided into seven groups as follows: Normal control, ISO, ISO + LY294002 (PI3K inhibitor), DATS+ISO, CAP+ISO, DATS+LY294002 + ISO, and CAP+LY294002 + ISO. All treatments (40 mg/kg DATS, 50 mg/kg CAP & 0.3 mg/kg LY294002) were given daily for two weeks before ISO injection (85 mg/kg for 2 days). At the end of the experiment, serum and cardiac tissues were collected. Serum cardiac troponin I (cTnI), and creatine kinase MB (CK-MB) were measured. Cardiac glutathione peroxidase (GSH-px), malondialdehyde (MDA), hypoxia-inducible factor 1 alpha (HIF-1α), autophagy proteins (P62 & LC3IIB) and gene expression of PI3K, Akt, FOXO-1, and eNOS were assessed. Histopathological examination of heart tissue was performed.ResultsDATS and CAP significantly (p < 0.01) decreased serum CK-MB and cTnI, cardiac levels of MDA, HIF-1α, p62 and LC3IIB along with an increase in GSH-px activity compared with ISO group. Moreover, DATS and CAP significantly up-regulated PI3K, Akt, and eNOS gene expression but down-regulated FOXO-1 expression compared to ISO group. However, LY294002 reversed DATS and CAP cardioprotective effects.ConclusionDATS and CAP prior treatment proved cardioprotective effects via modulation of autophagy, PI3K/Akt signaling, eNOS and FOXO-1 downregulation in ISO induced AMI rat model.

【 授权许可】

CC BY   
© The Author(s) 2022

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