Effect of ginsenoside compound K on alleviating colitis via modulating gut microbiota

Chinese Medicine

Research

Li Wang¹  Wei-Hua Huang¹  Man-Yun Chen¹  Lin Wang¹  Wei Zhang¹  Feng-Bo Tan²  Li Shao³ 

[1] Department of Clinical Pharmacology, Xiangya Hospital, Central South University, 410008, Changsha, China;Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, 410078, Changsha, China;National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, 410008, Changsha, China;Department of General Surgery, Xiangya Hospital, Central South University, 410008, Changsha, China;Department of Pharmacognosy, School of Pharmacy, Hunan University of Chinese Medicine, 410128, Changsha, China;

关键词: Ginsenoside compound K;    Colitis;    FMT;    Gut microbiota;   

DOI  :  10.1186/s13020-022-00701-9

 received in 2022-10-07, accepted in 2022-12-14,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundGinsenoside compound K (GC-K) potentially alleviates ulcerative colitis involved in gut microbiota, which is significantly associated with the occurrence and development of colitis. However, the effect and mechanism of GC-K on anti-colitis in relation to gut microbiota are not clear. This study focused on the prevention and mechanism of GC-K on Dextran sulfate sodium (DSS)-induced colitis of mice pertinent to gut microbiota.MethodsDSS was used to establish a chronic colitis mouse model. Body weight analysis, colon length measurement, HE staining, and inflammatory factors levels were processed in animal experiments. Flow cytometry was employed to analyze Th17/Treg cells in the mouse spleen and blood. 16S rRNA sequencing was utilized to analyze gut microbiota. Fecal microbiota transplantation (FMT) experiment was employed to verify the anti-colitis efficacy of GC-K by reshaping gut microbiota.ResultsGC-K significantly relieved colitis-related symptoms due to decreased disease activity index (DAI) scores, spleen weight, and increased colon length. Additionally, the tight junction proteins were increased, and the pro-inflammatory cytokines, such as TNF-α, IL-6, IL-1β and IL-17, were decreased after GC-K treatment. Furthermore, Bacteroides spp. significantly increased after modeling. Moreover, FMT experiments confirmed that GC-K-driven gut microbiota greatly relieved DSS-induced colitis.ConclusionGC-K alleviated colitis via the modulation of gut microbiota.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2022

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