BMC Research Notes | |
Clinically relevant benzoxaboroles inhibit mRNA processing in Trypanosoma brucei | |
Research Note | |
Albina Waithaka1  Christine Clayton1  | |
[1] Heidelberg University Centre for Molecular Biology (ZMBH), Im Neuenheimer Feld 282, D69120, Heidelberg, Germany; | |
关键词: CPSF73; Benzoxaboroles; Tandem affinity purification; mRNA processing; | |
DOI : 10.1186/s13104-022-06258-y | |
received in 2022-07-15, accepted in 2022-11-30, 发布年份 2022 | |
来源: Springer | |
【 摘 要 】
ObjectiveThe cleavage and polyadenylation endonuclease CPSF73 is thought to be the target of the anti-trypanosomal benzoxaboroles AN7973, acoziborole and AN11736. We previously showed that AN7973 inhibits mRNA processing. We here investigated whether the drug candidates acoziborole (for human sleeping sickness) and AN11736 (for nagana in cattle) have the same effect. We also affinity purified tagged CPSF73 from parasites without, or after, AN7973 treatment, and analysed differentially co-purified proteins by mass spectrometry.ResultsAN11736 and acoziborole both inhibited mRNA processing, as demonstrated by decreased levels of spliced mRNAs and accumulation of di- and tri-cistronic mRNAs from the alpha-beta tubulin locus. Treating the cells with AN7973 for 30 min. did not significantly affect the proteins that copurified with CPSF73.
【 授权许可】
CC BY
© The Author(s) 2022
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202305063328040ZK.pdf | 1338KB | download | |
12982_2022_119_Article_IEq86.gif | 1KB | Image | download |
12982_2022_119_Article_IEq234.gif | 1KB | Image | download |
12888_2022_4365_Article_IEq36.gif | 1KB | Image | download |
MediaObjects/12888_2022_4365_MOESM1_ESM.xlsx | 146KB | Other | download |
12982_2022_119_Article_IEq152.gif | 1KB | Image | download |
【 图 表 】
12982_2022_119_Article_IEq152.gif
12888_2022_4365_Article_IEq36.gif
12982_2022_119_Article_IEq234.gif
12982_2022_119_Article_IEq86.gif
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]