Repair of osteochondral defect using icariin-conditioned serum combined with chitosan in rabbit knees

BMC Complementary Medicine and Therapies

Research Article

Dong Ming¹  Juntao Zhang²  Chao Zhang³  Aifeng Liu³  Jianjie Jiao⁴  Man Shang⁴  Qiang Ji⁵ 

[1] Academy of Medical Engineering and Translational Medicine, Tianjin University, 92 Weijin Road, Nankai district, Tianjin, China;Academy of Medical Engineering and Translational Medicine, Tianjin University, 92 Weijin Road, Nankai district, Tianjin, China;Department of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, 88 Changling Road, Xiqing district, Tianjin, China;Department of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, 88 Changling Road, Xiqing district, Tianjin, China;Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District, Tianjin, China;Tianjin University of Traditional Chinese Medicine, 10 Boyanghu Road, Jinghai district, Tianjin, China;

关键词: Cartilage defect;    Chondrocyte;    Icarrin;    Chitosan;   

DOI  :  10.1186/s12906-020-02996-3

 received in 2020-02-04, accepted in 2020-06-16,  发布年份 2020

来源: Springer

【 摘 要 】

BackgroundOsteochondral defects caused by an acute traumatic injury or articular degeneration remains difficult to be manipulated. Repair of articular defects is still a great challenge for both tissue engineers and orthopedic surgeons. Therefore, combination of biomaterials with cartilage promotive drugs is well worth being developed to support the regeneration of both cartilage and subchondral bone.MethodsRabbits undergoing osteochondral defect surgery were intrarticularly injected with icariin-conditioned serum (ICS), chitosan (CSSH) and combination of ICS with CSSH, respectively. Gait analysis was performed using VICON motion capture system. ICRS score and immunohistochemical (IHC) analysis including H&E, Safranin O, toluidine blue and collagen II staining was employed to evaluate macroscopic cartilage regeneration and determine the morphologic repair of cartilage.ResultsRabbits with the treatment of ICS or CSSH alone showed mild improvement in hopping time and range of joint angles while ICS-CSSH group exhibited longer jumping time and larger range of joint angles. In addition, femoral condyle in ICS-CSSH rabbits could be seen with more native cartilage and subchondral bone regeneration in both macroscopic observation and IHC analysis.ConclusionICS combined with CSSH could promote the repair of osteochondral defect in rabbit knees. Combination of biomaterials with cartilage promotive drugs may ultimately have profound implications in the management of cartilage defect.

【 授权许可】

CC BY   
© The Author(s) 2020. corrected publication 2022

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