Integrated metabolomic and transcriptomic analyses of regulatory mechanisms associated with uniconazole-induced dwarfism in banana

BMC Plant Biology

Research

Liuyan Qin¹  Chaosheng Li¹  Shaolong Wei¹  Zhangfei He¹  Dandan Tian¹  Baoshen Li¹  Di Wei¹  Sumei Huang¹  Wei Zhou¹  Liping Wei¹  Shengfeng Long¹  Chenglin Guo² 

[1] Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, 530007, Nanning, China;Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences/National Local Joint Engineering Research Center for Genetic Improvement and Cultivation Techniques of Banana Varieties/National Tropical Fruit Variety improvement Center Guangxi Banana Branch Center, 530007, Nanning, China;Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, 530007, Nanning, China;

关键词: Banana dwarfing;    Uniconazole;    Metabolome;    Transcriptome;    Flavonoid synthesis;   

DOI  :  10.1186/s12870-022-04005-w

 received in 2022-08-12, accepted in 2022-12-14,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundUniconazole is an effective plant growth regulator that can be used in banana cultivation to promote dwarfing and enhance lodging resistance. However, the mechanisms underlying banana dwarfing induced by uniconazole are unknown. In uniconazole-treated bananas, gibberellin (GA) was downregulated compared to the control groups. An integrative analysis of transcriptomes and metabolomes was performed on dwarf bananas induced by uniconazole and control groups. The key pathways involved in uniconazole-induced dwarfism in banana were determined according to the overlap of KEGG annotation of differentially expressed genes and (DEGs) differential abundant metabolites (DAMs).ResultsCompared with the control groups, the levels of some flavonoids, tannins, and alkaloids increased, and those of most lipids, amino acids and derivatives, organic acids, nucleotides and derivatives, and terpenoids decreased in uniconazole-treated bananas. Metabolome analysis revealed the significant changes of flavonoids in uniconazole-treated bananas compared to control samples at both 15 days and 25 days post treatment. Transcriptome analysis shows that the DEGs between the treatment and control groups were related to a series of metabolic pathways, including lignin biosynthesis, phenylpropanoid metabolism, and peroxidase activity. Comprehensive analysis of the key pathways of co-enrichment of DEGs and DAMs from 15 d to 25 d after uniconazole treatment shows that flavonoid biosynthesis was upregulated.ConclusionsIn addition to the decrease in GA, the increase in tannin procyanidin B1 may contribute to dwarfing of banana plants by inhibiting the activity of GA. The increased of flavonoid biosynthesis and the change of lignin biosynthesis may lead to dwarfing phenotype of banana plants. This study expands our understanding of the mechanisms underlying uniconazole-induced banana dwarfing.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

13690_2022_1004_Article_IEq3.gif

Fig. 1

12982_2022_119_Article_IEq5.gif

12902_2022_1242_Article_IEq1.gif

13068_2022_2229_Article_IEq4.gif

13068_2022_2229_Article_IEq2.gif

Fig. 1