Omics analyses of Rehmannia glutinosa dedifferentiated and cambial meristematic cells reveal mechanisms of catalpol and indole alkaloid biosynthesis

BMC Plant Biology

Research

Pengfei Zhou¹  Haihua Li²  Yinzi Chen³  Xiaoqi Qiu³  Hui Yan³  Yujun Zhou³  Xuan Li³  Huiting Deng³  Yiheng Li³  Yujin Lin³  Beilu Xu³  Weiming Lin³ 

[1] School of Basic Medical Science, Guangdong Medical University, 523808, Dongguan, China;School of Medicine and Health, Guangdong Innovative Technical College, 523946, Dongguan, China;School of Pharmacy, Guangdong Medical University, 523808, Dongguan, China;

关键词: Cell suspension cultures;    Cambial meristematic cells;    Catalpol;    Iridoids;    Metabolome;    Transcriptome;    Plant-hormone signal transduction;    Tryptophan;    Phenylalanine;   

DOI  :  10.1186/s12870-023-04478-3

 received in 2022-12-09, accepted in 2023-09-19,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundRehmannia glutinosa is a rich source of terpenoids with a high medicinal reputation. The present study compared dedifferentiated cells (DDCs) and cambial meristematic cells (CMCs) cell cultures of R. glutinosa for terpenoid (catalpol) and indole alkaloid (IA) biosynthesis. In this regard, we used widely targeted metabolomics and transcriptome sequencing approaches together with the comparison of cell morphology, cell death (%), and catalpol production at different time points.ResultsWe were able to identify CMCs based on their morphology and hypersensitivity to zeocin. CMCs showed higher dry weight content and better catalpol production compared to DDCs. The metabolome analysis revealed higher concentrations of IA, terpenoids, and catalpol in CMCs compared to DDCs. The transcriptome sequencing analysis showed that a total of 27,201 genes enriched in 139 pathways were differentially expressed. The higher catalpol concentration in CMCs is related to the expression changes in genes involved in acetyl-CoA and geranyl-PP biosynthesis, which are precursors for monoterpenoid biosynthesis. Moreover, the expressions of the four primary genes involved in monoterpenoid biosynthesis (NMD, CYP76A26, UGT6, and CYP76F14), along with a squalene monooxygenase, exhibit a strong association with the distinct catalpol biosynthesis. Contrarily, expression changes in AADC, STR, and RBG genes were consistent with the IA biosynthesis. Finally, we discussed the phytohormone signaling and transcription factors in relation to observed changes in metabolome.ConclusionsOverall, our study provides novel data for improving the catalpol and IA biosynthesis in R. glutinosa.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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