Astragaloside IV attenuates IL-1β-induced intervertebral disc degeneration through inhibition of the NF-κB pathway

Journal of Orthopaedic Surgery and Research

Research Article

Weimin Deng¹  Yueyang Tian²  Xing Guo²  Qia Huang²  Yuan Xue²  Xu Chu³ 

[1] Department of Immunology, Tianjin Medical University, Tianjin, China;Department of Orthopedics Surgery, Tianjin Medical University General Hospital, Tianjin, China;Tianjin Key Laboratory of Spine and Spinal Cord Injury, Tianjin, China;Honghui Hospital, Xi’an Jiaotong University, Xi’an, China;

关键词: Astragaloside IV;    Intervertebral disc degeneration;    NF-κB;    Extracellular matrix;    Inflammation;    Apoptosis;   

DOI  :  10.1186/s13018-022-03438-1

 received in 2022-03-24, accepted in 2022-12-05,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIntervertebral disc degeneration (IDD) is the main cause of low back pain. Patients with low back pain may experience significant socio-economic burdens and decreased productivity. Previous studies have shown that inflammation is one of the main causes of IDD. Astragaloside IV (AS IV), a traditional Chinese medicine, has been reported to have therapeutic effects on many inflammation-related diseases; however, the effectiveness of AS IV as the treatment for IDD has not been studied.MethodsNucleus pulposus (NP) cells from patients with IDD were used for the experiments. Cell counting kit 8 (CCK8) was used to evaluate the effect of AS IV on the viability of NP cells (NPCs). To mimic IDD in vitro, NPCs were divided into the following groups: control group, interleukin 1β (IL-1β) group, and AS IV + IL-1β group. To analyse the effect of AS IV on IL-1β-induced IDD, Western blotting, RT-qPCR, flow cytometry, and immunofluorescence assays were performed. To evaluate the effect of AS IV in vivo, a rat model of puncture-induced IDD was established.ResultsAS IV effectively alleviated IL-1β-induced inflammation, apoptosis, and extracellular matrix degeneration in NPCs. We also observed that AS IV decreased the IL-1β-induced phosphorylation of inhibitor of kappa B-alpha (p-IκBα) in the cytosol, and reduced nuclear translocation of NF-κB p65, indicating that AS IV inhibited the NF-κB pathway. Using the puncture-induced rat IDD model, our results showed that AS IV had a protective effect against the progression of IDD, suggesting that AS IV could alleviate IDD in vivo.ConclusionsOur results demonstrated that AS IV effectively alleviated IDD in vivo and in vitro, indicating that it could be used as a therapeutic to treat IDD.

【 授权许可】

CC BY   
© The Author(s) 2022

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