Modulation of gut microbiota, blood metabolites, and disease resistance by dietary β-glucan in rainbow trout (Oncorhynchus mykiss)

Animal Microbiome

Research

Mie Bech Lukassen¹  Simon Menanteau-Ledouble¹  Jeppe Lund Nielsen¹  Ulrike Rolle-Kampczyk²  Sven-Bastiaan Haange²  Martin von Bergen³  Jakob Skov⁴  Inger Dalsgaard⁵ 

[1]Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg East, Denmark

[2]Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research, UFZ, Permoserstr. 15, 04318, Leipzig, Germany

[3]Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research, UFZ, Permoserstr. 15, 04318, Leipzig, Germany

[4]German Centre for Integrative Biodiversity Research, (iDiv) Halle-Jena-Leipzig, Puschstraße 4, 04103, Leipzig, Germany

[5]Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig, Brüderstraße 34, 04103, Leipzig, Germany

[6]Department of Veterinary and Animal Sciences, University of Copenhagen, Grønnegårdsvej 15, 1870, Frederiksberg C, Denmark

[7]National Institute of Aquatic Resources, Technical University of Denmark, Kemitorvet, 2800, Kongens Lyngby, Denmark

[8]National Institute of Aquatic Resources, Technical University of Denmark, Kemitorvet, 2800, Kongens Lyngby, Denmark

关键词: Oncorhynchus mykiss;    Gut microbiota;    β-glucan;    Prebiotic;    Metabolite response;   

DOI  :  10.1186/s42523-022-00209-5

 received in 2022-05-02, accepted in 2022-11-03,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundPrebiotics are known to have a positive impact on fish health and growth rate, and β-glucans are among the most used prebiotics on the market. In this study, rainbow trout (Oncorhynchus mykiss) were treated with a β-1,3;1,6-glucan dietary supplement (at a dose of 0 g, 1 g, 10 g, and 50 g β-glucan per kg of feed). After 6 weeks, the effect of the β-glucan was evaluated by determining the changes in the microbiota and the blood serum metabolites in the fish. The impact of β-glucan on the immune system was evaluated through a challenge experiment with the bacterial fish pathogen Yersinia ruckeri.ResultsThe microbiota showed a significant change in terms of composition following β-glucan treatment, notably an increase in the relative abundance of members of the genus Aurantimicrobium, associated with a decreased abundance of the genera Carnobacterium and Deefgea. Furthermore, analysis of more than 200 metabolites revealed that the relative levels of 53 metabolites, in particular compounds related to phosphatidylcholines, were up- or downregulated in response to the dietary supplementation, this included the amino acid alanine that was significantly upregulated in the fish that had received the highest dose of β-glucan. Meanwhile, no strong effect could be detected on the resistance of the fish to the bacterial infection.ConclusionsThe present study illustrates the ability of β-glucans to modify the gut microbiota of fish, resulting in alteration of the metabolome and affecting fish health through the lipidome of rainbow trout.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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