A novel organ culture model of a joint for the evaluation of static and dynamic load on articular cartilage

Bone & Joint Research

article

Y-C. Lin¹  A. C. Hall¹  A. H. R. W. Simpson¹ 

[1] University of Edinburgh

关键词: Joint organ culture;    Ex vivo model;    Dynamic movement;   

DOI  :  10.1302/2046-3758.73.BJR-2017-0320

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

ObjectivesThe purpose of this study was to create a novel ex vivo organ culture model for evaluating theeffects of static and dynamic load on cartilage.MethodsThe metatarsophalangeal joints of 12 fresh cadaveric bovine feet were skinned and dissected aseptically, and cultured for up to four weeks. Dynamic movement was applied usinga custom-made machine on six joints, with the others cultured under static conditions.Chondrocyte viability and matrix glycosaminoglycan (GAG) content were evaluated by thecell viability probes, 5-chloromethylfluorescein diacetate (CMFDA) and propidium iodide(PI), and dimethylmethylene blue (DMMB) assay, respectively.ResultsChondrocyte viability in the static model decreased significantly from 89.9% (sd 2.5%) (Day 0)to 66.5% (sd 13.1%) (Day 28), 94.7% (sd 1.1%) to 80. 9% (sd 5.8%) and 80.1% (sd 3.0%) to46.9% (sd 8.5%) in the superficial quarter, central half and deep quarter of cartilage, respectively (p < 0.001 in each zone; one-way analysis of variance). The GAG content decreased significantly from 6.01 μg/mg (sd 0.06) (Day 0) to 4.71 μg/mg (sd 0.06) (Day 28) (p < 0.001; one-wayanalysis of variance). However, with dynamic movement, chondrocyte viability and GAG content were maintained at the Day 0 level over the four-week period without a significant change(chondrocyte viability: 92.0% (sd 4.0%) (Day 0) to 89.9% (sd 0.2%) (Day 28), 93.1% (sd 1.5%)to 93.8% (sd 0.9%) and 85.6% (sd 0.8%) to 84.0% (sd 2.9%) in the three corresponding zones;GAG content: 6.18 μg/mg (sd 0.15) (Day 0) to 6.06 μg/mg (sd 0.09) (Day 28)).ConclusionDynamic joint movement maintained chondrocyte viability and cartilage GAG content. Thislong-term whole joint culture model could be of value in providing a more natural and controlled platform for investigating the influence of joint movement on articular cartilage, andfor evaluating novel therapies for cartilage repair.

【 授权许可】

CC BY-NC