HIV taken by STORM: Super-resolution fluorescence microscopy of a viral infection

Virology Journal

Katharina Gaus²  Johnson Mak¹  Dylan M Owen²  Jérémie Rossy²  Cândida F Pereira³ 

[1] Commonwealth Scientific and Industrial Research Organization, Australian Animal Health Laboratory, Geelong, Australia;Centre for Vascular Research, University of New South Wales, Sydney, Australia;Department of Medicine, Monash University, Clayton, Australia

关键词: Protein rearrangement;    Dynamic movement;    Viruses;    Electron microscopy;    Super-resolution microscopy;    HIV;   

Others  :  1154768
DOI  :  10.1186/1743-422X-9-84

 received in 2011-11-09, accepted in 2012-05-02,  发布年份 2012

【 摘 要 】

Background

The visualization of viral proteins has been hindered by the resolution limit of conventional fluorescent microscopes, as the dimension of any single fluorescent signal is often greater than most virion particles. Super-resolution microscopy has the potential to unveil the distribution of proteins at the resolution approaching electron microscopy without relying on morphological features of existing characteristics of the biological specimen that are needed in EM.

Results

Using direct stochastic optical reconstruction microscopy (dSTORM) to achieve a lateral resolution of 15–20 nm, we quantified the 2-D molecular distribution of the major structural proteins of the infectious human immunodeficiency virus type 1 (HIV-1) before and after infection of lymphoid cells. We determined that the HIV-1 matrix and capsid proteins undergo restructuring soon after HIV-1 infection.

Conclusions

This study provides the proof-of-concept for the use of dSTORM to visualize the changes in the molecular distribution of viral proteins during an infection.

【 授权许可】

   
2012 Pereira et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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