Cloning, Expression, and Characterization of a GHF 11 Xylanase from Alteromonas macleodii HY35 in Escherichia col i

The Journal of General and Applied Microbiology

article

Yanjie Tian¹  Jia Xu¹  Jianing Shi¹  Mengyuan Kong¹  Changjiang Guo¹  Caixia Cui¹  Yongtao Wang²  Yan Wang¹  Chenyan Zhou¹ 

[1] Synthetic Biology Engineering Laboratory of Henan Province, School of Life Science and Technology, Xinxiang Medical University;The First Affiliated Hospital, Xinxiang Medical University

关键词: Alteromonas macleodii HY35;    xylanase;    glycosyl hydrolase family11;    expression;    enzymaticproperties;   

DOI  :  10.2323/jgam.2021.10.003

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

A xylanase gene xynZT-1 from Alteromonas macleodiiHY35 was cloned and expressed in Escherichia coli(E. coli). The sequencing results showed that theORF of xynZT-1 was 831 bp. The xylanase DNAsequence encoded a 29 amino acids (aa) signal peptide and a 247-aa mature peptide. The XynZT-1 hasbeen a calculated molecular weight (MW) of 27.93kDa, isoelectric point (pI) of 5.11 and the formulaC1266H1829N327O384S5. The amino acid sequence ofthe xynZT-1 had a high similarity with that of glycosyl hydrolase family 11 (GHF11) reported fromother microorganisms. The DNA sequence encodingmature peptide was subcloned into pET-28a(+)expression vector. The resulted plasmid pET-28axynZT-1 was transformed into E. coli BL21(DE3),and the recombinant strain BL21(DE3)/xynZT-1 wasobtained. The optimum temperature and pH of therecombinant XynZT-1 were 45 ℃ and 5.0, respectively.

【 授权许可】

Unknown