Cytotoxic Effects of Artemisia Dracunculus L. and Heracleum Persicum Desf. Extracts on Leishmania major and Leishmania infantum Promastigotes Using MTT Assay

International Journal of Enteric Pathogens

article

Shahram Khademvatan¹  Kaveh Eskandari²  Batool Sadeghi Nejad³  Sedigheh Yusef Naanaie⁴ 

[1] Cellulor and Molecular Research Center, Department of Medical Parasitology and Mycology, Urmia University of Medical Sciences;Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapour University of Medical Sciences;Abadan University of Medical Sciences;The Agricultural and Natural Resource Center

关键词: Antileishmanial;    Leishmania major;    Leishmania infantum;    Promastigote;    Artemisia dracunculus;    Heracleum persicum;    MTT assay;   

DOI  :  10.34172/ijep.2021.12

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: Leishmaniasis is a parasitic disease that occurs in subtropical and tropical regions with approximately 350 million people worldwide and 2 million new cases annually. The annual increase in cutaneous leishmaniasis (CL) is observed, especially in endemic areas such as Iran. Since there is no effective vaccine, the detection of natural anti-leishmanial products is essential. Objective: The purpose of this study was to evaluate the in vitro anti-leishmanial activity of two herbal medicine including Artemisia dracunculu s L. and Heracleum persicum Desf. (Golpar). Materials and Methods: The extracts of selected plants were obtained by maceration, and in vitro anti-leishmanial activity was assayed on Leishmania major and Leishmania infantum promastigotes using colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay in comparison with glucantime as a reference. Results: Based on the results, 50% inhibitory concentration (IC50) values of selected plants and glucantime solutions were determined at 24, 48, and 72 hours incubation. Further, the anti-leishmanial activity of the leaf extract of A. dracunculus with IC50 values of 1.85 and 3.5 µg/mL and the fruit extract of H. persicum with values of 31.32 and 11.7 µg/mL were evaluated against L. major and L. infantum promastigotes, respectively. Conclusion: These results revealed anti-leishmania properties of the above-mentioned plants and the need to study the effects of these extracts on the Leishmania genus in animal models and in vivo assay in the future.

【 授权许可】

CC BY-NC