Evaluation of the Antileishmanial Properties of Ixorabrachiata Roxb on Leishmania major and Leishmaniainfantum by Colorimetric MTT Assay

International Journal of Enteric Pathogens

article

Kaveh Eskandari¹  Shahram Khademvatan²  Batool Sadeghi Nejad³  Sedigheh Yusef Naanaie⁴  Kobra kohansal⁵ 

[1] Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapour University of Medical Sciences;Department of Myco-Parasitology, Urmia University of Medical Sciences;Abadan Faculty of Medical Sciences;The Agricultural and Natural of Resources Center;Department of Medical Parasitology, School of Medicine, Jundishapur University of Medical Sciences

关键词: Antileishmanial;    Leishmania major;    Leishmania infantum;    Promastigote;    Ixora brachiata;    Colorimetric MTT;   

DOI  :  10.15171/ijep.2019.26

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: Leishmaniasis is a major global health problem which affects millions of people, especially in the developing countries. The incidence of leishmaniasis has increased and there is no vaccination for Leishmania infections and standard drugs for treatment of the disease have many side effects; therefore, it is necessary to find new effective alternatives. Objectives: The purpose of this study was to evaluate the in vitro antileishmanial activity of Ixora brachiata root extract against Leishmania major and Leishmania infantum promastigotes. Materials and Methods: Different doses of the selected plant extract was tested against L. major and L. infantum promastigotes using colorimetric MTT [3-(4, 5-methylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay. Glucantime was used as the positive control. Results: Anti-parasitic activity was revealed for I. brachiata root on L. major and L. infantum with 50% inhibitory concentration (IC50) values of 0.91 and 2.63 µg/mL, respectively compared to the standard drugs, glucantime, which had an IC50 value of 40.2 µg/mL for L. major and 18.5 µg/ mL for L. infantum after 72 hours. Conclusion: The results of this study created a new background on the development of drug against leishmania parasite.

【 授权许可】

CC BY-NC