Comparison of chondrogenesis induction between in vitro and animal models using TGF-β3, kartogenin and avocado/soybean

Jundishapur Scientific Medical Journal

article

Hashemibeni, Batool¹  Izadi, Mohammad Ali¹  Valiani, Ali¹  Esfandiari, Ebrahim¹  Bahramian, Hamid¹  Pourentezari, Majid² 

[1] Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences;Department of Biology and Anatomical Sciences, School of Basic Sciences, Shahid Sadoughi University of Medical Sciences

关键词: TGF-β3;    Kartogenin;    Avocado/Soybean;    Adipose-derived stem cells;    Fibrin;    Chondrogenesis;   

DOI  :  10.32598/JSMJ.21.2.2296

学科分类：基础医学

来源: Ahvaz Jundishapur University of Medical Sciences

【 摘 要 】

Background and Objectives Cartilage tissue engineering, by using stem cells, scaffolding and appropriategrowth factors, seek to produce natural cartilage tissue to replace damaged tissue and solve theproblems that exist in the treatment of cartilage damage. This study aims to compare chondrogenicdifferentiation of adipose-derived stem cells under the influence of the transforming growth factor beta3 (TGF-β3), Kartogenin, and avocado/soybean unsaponifiables (ASU) on the fibrin scaffold in the laboratoryand animal models.Subjects and Methods In this experimental laboratory study, after extraction of stem cells from humanadipose tissue, induction of chondrogenic differentiation was done for 14 days on the fibrin scaffold inlaboratory. The cells differentiated in the fibrin scaffold were transplanted subcutaneously under theskin of male rats for two weeks. Then, comparison of histological and immunohistochemical studies wasperformed in both laboratory and animal models.Results A significant increase in the density of Toluidine blue dye accumulation was observed in TGF-β3,Kartogenin and ASU groups in the animal model compared to the laboratory model. Immunohistochemicalresults for the collagen type X accumulation in the TGF-β3 group showed a significant increase in theanimal model compared to the laboratory model. In the Kartogenin and ASU groups, the accumulationof collagen type Χ showed a significant decrease in the animal model compared to the laboratory model.Conclusion The implantation of differentiated cartilage cells in laboratory before subcutaneous transferto the skin can help mature and complete the characteristics of the constructed cartilage.

【 授权许可】

Unknown