The Comparison of cefoxitin disk disffusion method with other phenotypic and molecular methods in identification of methicillin-resistant Staphylococcus aureus in clinical specimens of patients admitted to Ahvaz educational hospitals

Jundishapur Scientific Medical Journal

article

Hasanian, Soheila¹  Amir Mozafari, Nour²  Farajzadeh Sheikh, Ahmad³  Mehregan, Iraj⁴ 

[1] phd;Department of Microbiology, Iran University of Medical Sciences;Khuzestan Cochlear Implant Center;Islamic Azad University of Tehran Research Sciences

关键词: Staphylococcus aureus;    MRSA;    Antibiotic Resistance;    Genotype;   

DOI  :  10.22118/jsmj.2020.201706.1832

学科分类：基础医学

来源: Ahvaz Jundishapur University of Medical Sciences

【 摘 要 】

Background and Objective: Staphylococcus aureus is one of the few bacteria that can infect all organs of the body. The aim of this study was to evalluate the cefoxitin disk diffusion method with other phenotypic and molecular methods in the detection of methicillin-resistant Staphylococcus aureus designed and adjusted in clinical specimens of admitted patients in Ahvaz educational hospitals, thereby followed by S.aureus relative prevalence and antibiotic susceptibility determination. Subjects and Methods: A total of 280 S. aureus isolates from clinical specimens were collected. Tests were performed using Cefoxitin disc diffusion, Oxacillin screening agar and Oxacillin disk diffusion methods. The PCR method was used to determine the mec A gene. Results: 0ut of a total of 693 specimens, 280 specimens were confirmed as S. aureus, 123 strains of which were detected as MRSA. The comparison of Oxacillin disk diffusion and oxacillin screening methods is indicative of 121 (43.2%) and 122 (40.6%) antibiotic susceptibilities, respectively, while isolates of MRSA were detected by disc diffusion cefoxitin and PCR 123 (43.9%). In determining the antibiotic susceptibility pattern, erythromycin (98.3%), ciprofloxacin (97.5%), clindamycin (94.3%), tetracycline (90.2%), gentamicin (83.7%) and rifampin (41.4%) were noticed. Also, low resistance to teicoplanin (0%), linzolide (0%), vancomycin (0%) was observed. Conclusion: The prevalence of methicillin resistant Staphylococci in our region was relatively high, but these strains were totally sensitive to vancomycin, teicoplanin and and linzolide antibiotics and cefoxitin disk test was the reliable method for detection of methicillin resistant Staphylococci.

【 授权许可】

Unknown