Inhibition of voltage‑gated potassium channels affect expressions of miR-126 and miR-126* in breast cancer cell lines

Bratislava Medical Journal

article

D. Turgut Cosan¹  A. Soyocak²  C. Oner³ 

[1] Eskisehir Osmangazi University, Medical Faculty, Department of Medical Biology;Istanbul Aydin University, Medical Faculty, Department of Medical Biology and Genetics;Maltepe University, Medical Faculty, Department of Medical Biology and Genetics

关键词: breast cancer;    Kv1.3;    Kv10.1;    miR-126;    miR-126*;   

DOI  :  10.4149/BLL_2020_014

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

AIM: We aimed to determine the possible correlation between voltage‑gated potassium channels and micro RNAs in breast cancer and metastatic breast cancer cells. METHOD: Kv1.3 and Kv10.1 channels were inhibited by specific siRNAs using a lipofectamine-based transfection in MCF-7 and MDA-MB-231 cells. After transfection, total RNA was isolated, and then miR-126 and miR-126* expressions were observed using RT-PCR. RESULTS: There was a negative correlation between Kv channels and miRNAs according to the characteristics of the breast cancer cells. The inhibition was observed not only in Kv1.3 but also in Kv10.1 in MCF-7 cells, and miR-126 and miR-126* expressions were downregulated compared to the control group (p < 0.001). The inhibition of these channels in MDA-MB-231 cells caused an upregulation of miR-126 and miR-126* expressions (p < 0.001). CONCLUSION: The miR-126 and miR-126* expressions differed according to benign and malign breast cancer cell lines. Furthermore, we found that miR-126/126* may interact with Kv1.3 and Kv10.1 voltage-gated potassium channels. Our study suggests and indicates the relationship between Kv channels and miRNAs in breast cancer cells (Tab. 1, Fig. 2, Ref. 51).

【 授权许可】

CC BY