Low concentrations of morphine enhanced the neuroglia-like differentiation

Bratislava Medical Journal

article

H. Zhaleh¹  M. Azadbakht²  A. Bidmeshki Pour² 

[1] Substance Abuse Prevention Research Center, Kermanshah University of Medical Sciences;Department of Biology, Faculty of Science, Razi University

关键词: morphine;    naltrexone;    neuroglial differentiation;    PC12 cell.;   

DOI  :  10.4149/BLL_2020_041

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

AIM: The present study was undertaken to evaluate the effects of different concentrations of morphine in chronic manner on neuroglial differentiation in PC12 cells. METHODS: PC12 cells were cultured in RPMI 1640 culture medium including 0.02 % bovine serum albumin together with different concentrations of morphine for 12 days. Cytotoxicity was performed by lactate dehydrogenase assay. Cell death was performed by PI/Hoechst staining assay. Neuroglial differentiation was performed by Nestin, Tuj-1, MAP-2, S-100 and GFAP Immunocytochemistry assay. RESULTS: Data showed that morphine either at low or high concentration activated opioid  receptors, which  resulted in a decrease of cytotoxicity and cell death and induction of Nestin, Tuj-1, MAP-2, Neurofilament-M (NF-M), GFAP and S-100 protein expression as compared in treated cells  with the control (untreated cells) (p<005). CONCLUSION: It can be concluded that low concentrations of morphine in chronic manner stimulate the neuroglial-like differentiation by activating protein expression and survival-promoting signaling in PC12 cells with opioid receptor-dependent mechanism (Fig. 8, Ref. 36). Text in PDF www.elis.sk.

【 授权许可】

CC BY