Prevention Of Oral Epithelial Dysplasia And Neoplasya Using Guabiroba Extract: A Study With Wistar Rats

Australian Journal of Basic and Applied Sciences

article

Luis Noldin Junior ; Paulo César Amorim ; Sandra Denise Camargo Mendes ; Stefany Grützmann Arcari ; Sarah Freygang Mendes Pilati

关键词: Antioxidants;    Carcinogenesis;    Inflammation;    Squamous cell carcinoma;   

DOI  :  10.22587/ajbas.2022.16.10.2

学科分类：社会科学、人文和艺术（综合）

来源: AENSI

【 摘 要 】

BACKGROUND: In recent years, studies focused on the total antioxidant activity present in native fruits in Brazil due to the compounds present with properties of reducing the level of oxidative stress and consequently reducing the risk of several cardiovascular, neurodegenerative and cancer. Objective: The aim of the study was to analyze the effects of guabiroba fruit extract on chemically induced carcinogenesis in rats. METHODS: Carcinogenesis was chemically induced by the compound 9,10-dimethyl-1,2-benzanthracene in oral mucosa applications in Wistar rats using only the carcinogen in the control group, the gel vehicle, application of pure extract and for the experimental group, applications of 9,10-dimethyl-1,2-benzanthracene and topical application of guabiroba pulp extract in 24 rats divided into 4 groups. After 60 days, the animals were euthanized to collect tissue samples. The tissue samples were analyzed in a microscope using Hematoxylin Eosin coloration with World Health Organization criteria. RESULTS: Cases of moderate and intense dysplasia were found in 66.7% of the animals in the control group and 33.3% in the experimental group. In pure extract group and gel vehicle group animals, there were no cases of moderate and intense epithelial dysplasia. Inflammatory infiltrate was found in 100 % of the slides of the control group; in 33.3 % of the experimental group only and in 16. 7% of the pure extract group and the gel vehicle group. The results showed that there was a difference between the control and experimental groups, i.e., in the control group, which was only in contact with the 9,10-dimethyl-1,2-benzanthracene, dysplasia occurred in whole animals. In comparison, in the experimental group, in addition to the carcinogen, the guabiroba extract was also applied, and there were half as many samples with dysplasia. Conclusion:9,10-dimethyl-1,2-benzanthracene proved to be effective in promoting chemical carcinogenesis and should be used in rat animal models of oral cancer. The results found in this study showed that guabiroba fruit extract has the ability to reduce the formation of epithelial dysplasia, suggesting its preventive efficacy; in addition, it can reduce the presence of inflammatory infiltrate in oral mucosa.

【 授权许可】

Unknown