Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene

Stem cell research

article

Zehra Yildirim¹  Ana Kojic¹  Christopher D. Yan¹  Matthew A. Wu¹  Randall Vagelos²  Joseph C. Wu¹ 

[1] Stanford Cardiovascular Institute, Stanford University School of Medicine;Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine;Department of Radiology, Stanford University School of Medicine

关键词: Dilated cardiomyopathy;    iPSC;    Stem cells;    HCN4;   

DOI  :  10.1016/j.scr.2022.102951

学科分类：生理学

来源: Academic Press

【 摘 要 】

Dilated cardiomyopathy (DCM) is a progressive heart muscle disease that can culminate with heart failure and death. Mutations in several genes can cause DCM, including hyperpolarization-activated cyclic nucleotide-gated channel (HCN4), which has a critical function in the autonomic control of the heart rate. Here, we generated two human induced pluripotent stem cell (iPSC) lines generated from two DCM patients carrying variants in the HCN4 gene (c.2587G > T and c.2846G > A). Both lines display normal karyotype, typical morphology of pluripotent stem cells, and differentiate into all three germ layers in vitro . These lines are valuable resources for studying the pathological mechanisms of DCM.

【 授权许可】

CC BY|CC BY-NC-ND