Contractility detection of isolated mouse papillary muscle using myotronic Myostation-Intact device

Animal Models and Experimental Medicine

article

Hong Lian¹  Zhuyun Qin¹  Mengge Wu⁴  Peipei Zuo⁴  Lina Bai¹  Minjie Lu¹  Lulu Li¹  Haitao Zhang³ 

[1] State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College;Beijing Key Laboratory of Preclinical Research and Evaluation for Cardiovascular Implant Materials, Animal Experimental Center, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College;Adult Surgical Intensive Care Unit, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College;National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Fuwai Central-China Hospital, Central-China Subcenter of National Center for Cardiovascular Diseases

关键词: calcium concentration;    frequency;    isolated papillary muscle;    muscle contractility;    Myostation-intact;    voltage;   

DOI  :  10.1002/ame2.12272

学科分类：机械工程学

来源: Wiley

【 摘 要 】

Background To understand the relationship between myocardial contractility and external stimuli, detecting ex vivo myocardial contractility is necessary. Methods We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies, voltages, and calcium concentrations. Results The results indicated that the basal contractility of the papillary muscle was 0.27 ± 0.03 mN at 10 V, 500-ms pulse duration, and 1 Hz. From 0.1 to 1.0 Hz, contractility decreased with an increase in frequency (0.45 ± 0.11–0.10 ± 0.02 mN). The voltage-initiated muscle contractility varied from 3 to 6 V, and the contractility gradually increased as the voltage increased from 6 to 10 V (0.14 ± 0.02–0.28 ± 0.03 mN). Moreover, the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM (0.45 ± 0.17–1.11 ± 0.05 mN); however, the contractility stopped increasing even when the concentration was increased to 7.5 mM (1.02 ± 0.23 mN). Conclusions Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND