Refining murine heterotopic heart transplantation: A model to study ischemia and reperfusion injury in donation after circulatory death hearts

Animal Models and Experimental Medicine

article

Mohammed Quader¹  Renee Cholyway¹  Niluka Wickramaratne¹  Oluwatoyin Akande¹  Martin Mangino¹  Eleonora Mezzaroma²  Adolfo G. Mauro³  Qun Chen³  Alexander Kantlis¹  Stefano Toldo³ 

[1] Division of Cardiothoracic Surgery, Pauley Heart Center, Virginia Commonwealth University;School of Pharmacy, Virginia Commonwealth University;Division of Cardiology, Pauley Heart Center, Virginia Commonwealth University

关键词: animal models;    cardiovascular biology;    cardiovascular disorders;    reproducibility;   

DOI  :  10.1002/ame2.12176

学科分类：机械工程学

来源: Wiley

【 摘 要 】

Heart transplantation is a lifesaving procedure, which is limited by the availability of donor hearts. Using hearts from donors after circulatory death, which have sustained global ischemia, requires thorough studies on reliable and reproducible models that developing researchers may not have mastered. By combining the most recent literature and our recommendations based on observations and trials and errors, the methods here detail a sound in vivo heterotopic heart transplantation model for rats in which protective interventions on the ischemic heart can be studied, and thus allowing the scientific community to advance organ preservation research. Knowledge gathered from reproducible animal models allow for successful translation to clinical studies.

【 授权许可】

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