Oxidative modifications and structural changes of human serum albumin in response to air dielectric barrier discharge plasma

High voltage

article

Siqi Peng¹  Liyang Zhang¹  Dongheyu Zhang¹  Yuntao Guo¹  Xinxin Wang¹  Qun Zhou²  Haiyun Luo¹ 

[1] Department of Electrical Engineering, Tsinghua University;Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology ,(Ministry of Education), Tsinghua University

关键词: biochemistry;    electrophoresis;    Fourier transform spectra;    infrared spectra;    molecular biophysics;    molecular configurations;    oxidation;    proteins;   

DOI  :  10.1049/hve2.12111

学科分类：物理（综合）

来源: Wiley

【 摘 要 】

In this work, the air dielectric barrier discharge plasma was applied to a model of protein human serum albumin to explore plasma-mediated protein damage from a biomolecular perspective. Carbonylation and side chain modifications of protein in solution were observed by Fourier transform infrared (FT-IR) spectroscopy and mass spectrometry (MS). Protein backbone cleavage is proved by Bradford assay, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, and MS. Typical fragments are in the range of 10–12 kDa. The decrease in α -helix and increase in β -sheet indicated by FT-IR are major protein secondary structure changes. Accordingly, a whole map of protein oxidative damage and structural changes in response to the plasma was postulated.

【 授权许可】

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