LIM protein Ajuba promotes liver cell proliferation through its involvement in DNA replication and DNA damage control

FEBS Letters

article

Noëlle Dommann¹  Jacopo Gavini¹  Daniel Sánchez-Taltavull¹  Felix Alexander Baier¹  Fabienne Birrer¹  Giulio Loforese¹  Daniel Candinas¹  Deborah Stroka¹ 

[1] Department of Visceral Surgery and Medicine, Inselspital, Bern University Hospital, University of Bern

关键词: Ajuba;    DNA damage;    liver cancer;    liver regeneration;    proliferation;   

DOI  :  10.1002/1873-3468.14371

来源: John Wiley & Sons Ltd.

【 摘 要 】

The LIM-domain protein Ajuba is associated with cell proliferation, a fundamental process of tissue regeneration and cancer. We report that in the liver, Ajuba expression is increased during regeneration and in tumour cells and tissues. Knockout of Ajuba using CRISPR/Cas9 is embryonic lethal in mice. shRNA targeting of Ajuba reduces cell proliferation, delays cell entry into S-phase, reduces cell survival and tumour growth in vivo and increases expression of the DNA damage marker γH2AX. Ajuba binding partners include proteins involved in DNA replication and damage, such as SKP2, MCM2, MCM7 and RPA70. Taken together, our data support that Ajuba promotes liver cell proliferation associated with development, regeneration and tumour growth and is involved in DNA replication and damage repair.

【 授权许可】

Unknown