FEBS Letters | |
PACS-1 contains distinct motifs for nuclear-cytoplasmic transport and interacts with the RNA-binding protein PTBP1 in the nucleus and cytosol | |
article | |
Steven M. Trothen1  Rong Xuan Zang1  Antony Lurie1  Jimmy D. Dikeakos1  | |
[1] Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, University of Western Ontario | |
关键词: IPO5/KPNA1; nuclear export signal; nuclear localization signal; nuclear transport; PACS-1; protein–protein interaction; PTBP1; RNA-binding protein; XPO1; | |
DOI : 10.1002/1873-3468.14243 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Phosphofurin acidic cluster sorting protein 1 (PACS-1) is canonically a cytosolic trafficking protein, yet recent reports have described nuclear roles for PACS-1. Herein, we sought to define the nuclear transport mechanism of PACS-1. We demonstrate that PACS-1 nucleocytoplasmic trafficking is dependent on its interaction with the nuclear transport receptors importin alpha 5 and exportin 1. PACS-1 nuclear entry and exit are defined by a nuclear localization signal (NLS, residues 311-318) and nuclear export signal (NES3, residues 366-375). Mutation of the PACS-1 NLS and NES3 altered the localization of a complex formed between PACS-1 and an RNA-binding protein, polypyrimidine tract-binding protein 1. Overall, we identify the nuclear localization mechanism of PACS-1 and highlight a potential role for PACS-1 in RNA-binding protein trafficking.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202302050002176ZK.pdf | 3091KB | download |