Bacterial Growth Inhibition Screen (BGIS): harnessing recombinant protein toxicity for rapid and unbiased interrogation of protein function

FEBS Letters

article

Haihong Guo¹  Nengwei Xu²  Malte Prell¹  Hiltrud Königs³  Benita Hermanns-Sachweh³  Bernhard Lüscher¹  Ferdinand Kappes¹ 

[1] Institute for Biochemistry and Molecular Biology, Medical School, RWTH Aachen University;Department of Biological Sciences, Suzhou Dushu Lake Science and Education Innovation District, Suzhou Industrial Park, Xi’an Jiaotong-Liverpool University;Institute of Pathology, Medical School, RWTH Aachen University

关键词: DEK;    Escherichia coli;    oncogene;    protein domain;    protein function;    recombinant protein toxicity;    RNA-binding domain;   

DOI  :  10.1002/1873-3468.14072

来源: John Wiley & Sons Ltd.

【 摘 要 】

In two proof-of-concept studies, we established and validated the Bacterial Growth Inhibition Screen (BGIS), which explores recombinant protein toxicity in Escherichia coli as a largely overlooked and alternative means for basic characterization of functional eukaryotic protein domains. By applying BGIS, we identified an unrecognized RNA-interacting domain in the DEK oncoprotein (this study) and successfully combined BGIS with random mutagenesis as a screening tool for loss-of-function mutants of the DNA modulating domain of DEK [1]. Collectively, our findings shed new light on the phenomenon of recombinant protein toxicity in E. coli . Given the easy and rapid implementation and wide applicability, BGIS will extend the repertoire of basic methods for the identification, analysis and unbiased manipulation of proteins.

【 授权许可】

Unknown