The Saccharomyces cerevisiae ABC subfamily D transporter Pxa1/Pxa2p co-imports CoASH into the peroxisome

FEBS Letters

article

Carlo W. T. van Roermund¹  Lodewijk IJlst¹  Alison Baker²  Ronald J. A. Wanders¹  Freddie L. Theodoulou³  Hans R. Waterham¹ 

[1] Laboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology & Metabolism, Amsterdam UMC, University of Amsterdam;Centre for Plant Sciences, School of Molecular and Cellular Biology, University of Leeds;Plant Sciences Department, Rothamsted Research

关键词: ABC transporter;    acyl-CoA;    coenzyme A;    coenzyme homeostasis;    peroxisome;    thioesterase;   

DOI  :  10.1002/1873-3468.13974

来源: John Wiley & Sons Ltd.

【 摘 要 】

ATP-binding cassette (ABC) subfamily D transporters are important for the uptake of fatty acids and other beta-oxidation substrates into peroxisomes. Genetic and biochemical evidence indicates that the transporters accept fatty acyl-coenzyme A that is cleaved during the transport cycle and then re-esterified in the peroxisomal lumen. However, it is not known whether free coenzyme A (CoA) is released inside or outside the peroxisome. Here we have used Saccharomyces cerevisiae and isolated peroxisomes to demonstrate that free CoA is released in the peroxisomal lumen. Thus, ABC subfamily D transporter provide an import pathway for free CoA that controls peroxisomal CoA homeostasis and tunes metabolism according to the cell’s demands.

【 授权许可】

Unknown