Reproductive medicine and biology | |
Usefulness of expanding the indications of early rescue intracytoplasmic sperm injection | |
article | |
Takashi Shibahara1  Yuu Fukasaku1  Nozomi Miyazaki1  Hiroaki Kawato2  Hiroyuki Minoura1  | |
[1] Minoura Ladies Clinic;Kawato Ladies Clinic | |
关键词: blastocyst; fertilization; intracytoplasmic sperm injection; oocyte; polar body; | |
DOI : 10.1002/rmb2.12432 | |
学科分类:工业工程学 | |
来源: Wiley | |
【 摘 要 】
Purpose Early rescue intracytoplasmic sperm injection (ICSI) is often performed in cases in which not even a single oocyte has extruded a second polar body 6 h after insemination. We evaluated the usefulness of expanding the indications of early rescue ICSI to cases in which <80% of oocytes have extruded second polar bodies 6 h after insemination. Methods Early rescue ICSI was performed on oocytes that were denuded 2.5 h post-insemination and whose extrusion of the second polar bodies had been examined 6 h post-insemination with a PolScope. Results In vitro fertilization was performed on 24 496 oocytes of 4944 cycles, and 1438 cycles had <80% rate of the second polar body extrusion. Rescue ICSI was performed on 3933 oocytes. Three pronuclei (3PN) incidence of rescue ICSI was 3.0% in oocytes with ≥50% rate of the second polar body extrusion. With respect to the second polar body extrusion rate, no differences were observed in normal fertilization, blastocyst development, implantation, miscarriage, or live birth rates for rescue ICSI. Conclusion By expanding the indications of early rescue ICSI using the PolScope to cases in which <80% of oocytes have extruded the second polar bodies, many fertilized oocytes can be obtained without considerably increasing the 3PN rate.
【 授权许可】
CC BY|CC BY-NC|CC BY-NC-ND
【 预 览 】
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RO202302050001933ZK.pdf | 789KB | download |