Meiotic spindle size is a strong indicator of human oocyte quality

Reproductive Medicine and Biology

卷:17

Toshitaka Horiuchi¹  Kayoko Nishimura²  Naomi Hidaka²  Hiroyuki Tomari²  Saori Kuhara²  Natsumi Aramaki²  Yumi Nagata²  Katsuko Kunitake²  Ko Honjo² 

[1] Graduate School of Comprehensive Scientific Research Prefectural University of Hiroshima Hiroshima Japan;

[2] IVF Nagata Clinic Fukuoka Japan;

关键词: assisted reproductive technology;    blastocyst;    fertilization;    intracytoplasmic sperm injection;    meiotic spindle;   

DOI  :  10.1002/rmb2.12100

来源: DOAJ

【 摘 要 】

Abstract Purpose To investigate the relationship between the meiotic spindle size in human metaphase II oocytes and embryo developmental potential after intracytoplasmic sperm injection (ICSI). Methods Analyzed were 1302 oocytes with a visible meiotic spindle from 281 patients aged under 40 years undergoing ICSI cycles. The meiotic spindle was imaged by using PolScope before ICSI. The oocytes were classified into three groups, according to spindle size: group A (<90 μm2), group B (90‐120 μm2), and group C (>120 μm2). Results Overall, 389 (29.9%) oocytes were classified into group A, 662 (50.8%) into group B, and 251 (19.3%) into group C. The fertilization rate of the group B oocytes was significantly higher than for the A and C oocytes. The blastocyst formation rate in group B was significantly higher than in group A. In addition, the pregnancy rate in group B was significantly higher than in the other two groups. Conclusion The oocytes with a spindle size of 90‐120 μm2 showed higher fertilization, blastocyst formation, and clinical pregnancy rates than those with larger or smaller spindles. The measurement of the meiotic spindle size thus has a positive predictive value for identifying human embryo developmental potential clinically.

【 授权许可】

Unknown