Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified-thawed human ovarian tissue xenografted to nude mice.

Reproductive medicine and biology

article

Kanako Matsumoto¹  Ayumi Harada¹  Kiyonori Miura²  Michio Kitajima²  Naoko Murakami¹  Yuriko Kitajima¹  Itsuki Kajimura¹  Asako Matsumura¹ 

[1] Department of Obstetrics and Gynecology, Nagasaki University Hospital;Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences

关键词: alpha-smooth muscle actin;    fibrosis;    follicular growth;    ovarian cortical stroma;    ovarian tissue cryopreservation;   

DOI  :  10.1002/rmb2.12451

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Purpose Ovarian tissue cryopreservation and its auto-transplantation is promising technique in fertility preservation. Longevity of grafted tissue is limited though mechanism of follicle reduction is not fully understood. We evaluated histological alteration of vitrified-thawed ovarian tissue that grafted to nude mice. Materials and Methods Human ovarian tissue was cryopreserved by vitrification. After thawing, they were grafted to mesentery of nude mice. Twelve weeks after transplantation, the implants were removed and histologically examined. The presence of follicles, the degree of fibrosis, and TUNEL staining in surrounding cortex were evaluated. The stromal expressions of alpha-smooth muscle actin (aSMA) were determined. Results Normal ovarian cortex was decreased, and fibrotic area were significantly increased after grafting. The distributions of developmental stage of follicles shifted toward activation of follicular growth. Stromal TUNEL staining was increased in frozen/thawed tissue. The expression of aSMA were found in perifollicular stroma of growing follicles, which were decreased in grafted tissue associated with reduction of cortical stroma. Conclusions Fibrosis, reduced cortical stroma, and activation of dormant follicles were concomitantly observed in grafted ovarian cortex, which may relate to limited longevity. Perifollicular aSMA expression can be regarded as a marker of the competence of cortical stroma that regulate follicular development.

【 授权许可】

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