Necropsy Validation of a Novel Method for Left Ventricular Mass Quantification in Porcine Transthoracic and Transdiaphragmal Echocardiography

Frontiers in Cardiovascular Medicine

article

Charlotte Burup Kristensen¹  Stefan Michael Sattler¹  Anniek Frederike Lubberding²  Jacob Tfelt-Hansen¹  Thomas Jespersen²  Christian Hassager¹  Rasmus Mogelvang¹ 

[1] Department of Cardiology, The Heart Center Rigshospitalet;Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen;Department of Forensic Genetics, Faculty of Health and Medical Sciences, University of Copenhagen;Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen;Cardiovascular Research Unit, University of Southern Denmark

关键词: left ventricular mass;    echocardiography;    left ventricular hypertrophy;    necropsy;    animal model;   

DOI  :  10.3389/fcvm.2022.868603

学科分类：地球科学(综合)

来源: Frontiers

【 摘 要 】

Introduction Increased left ventricular mass (LVM) is one of the most powerful predictors of adverse cardiovascular events. Clinical evaluation requires reliable, accurate and reproducible echocardiographic LVM-quantification to manage patients. For this purpose, we have developed a novel two-dimensional (2D) method based on adding the mean wall thickness to the left ventricular volume acquired by the biplane method of disks, which has recently been validated in humans using cardiac magnetic resonance as reference value. We assessed the hypothesis that the novel method has better accuracy than conventional one-dimensional (1D) methods, when compared to necropsy LVM in pigs. Materials and Methods Echocardiography was performed during anesthesia in 34 Danish Landrace pigs, weight 47–59 kg. All pigs were euthanized, cardiac necropsy was performed and the left ventricle was trimmed and weighed for necropsy LVM. Trans-thoracic echocardiography was applied for parasternal images. Transdiaphragmal echocardiography was applied for the apical images, which are otherwise difficult to obtain in pigs. We compared the conventional 1D- and 2D-methods and the novel 2D-method to the LVM from cardiac necropsy. Results Necropsy LVM was 132 ± 11 g (mean ± SD). The novel method had better accuracy than other methods (mean difference ± 95% limits of agreement; coefficients of variation; standard error of the estimate, Pearson's correlation). Novel (−1 ± 20 g; 8%; 11 g; r = 0.70), Devereux (+26 ± 37 g; 15%; 33 g; r = 0.52), Area-Length (+27 ± 34 g; 13 %; 33 g; r = 0.63), Truncated Ellipsoid (+10 ± 30 g; 12%; 19 g; r = 0.63), biplane endo-/epicardial tracing (−3 ± 2 g; 10%; 14 g; r = 0.57). No proportional bias in linear regression was detected for any method, when compared to necropsy LVM. Conclusion We confirm high accuracy of the novel 2D-based method compared to conventional 1D/2D-methods.

【 授权许可】

CC BY