The Particular Expression Profiles of Circular RNA in Peripheral Blood of Myocardial Infarction Patients by RNA Sequencing

Frontiers in Cardiovascular Medicine

article

Qi Li¹  Yuanyong Wang⁴  Yi An⁵  Jianxun Wang³  Yufang Gao⁶ 

[1] School of Nursing, Qingdao University;Department of Nursing, The Affiliated Hospital of Qingdao University;School of Basic Medical Sciences, Qingdao University;Department of Thoracic Surgery, Tangdu Hospital of Air Force Military Medical University;Department of Cardiology, The Affiliated Hospital of Qingdao University;Department of Emergency, The Affiliated Hospital of Qingdao University

关键词: circular RNA;    myocardial infarction;    RNA sequencing;    biomarkers;    cardiovascular diseases;   

DOI  :  10.3389/fcvm.2022.810257

学科分类：地球科学(综合)

来源: Frontiers

【 摘 要 】

Myocardial infarction (MI) is one of the most common illnesses seriously harmful to human health. Notwithstanding, the systems of its pathogenesis are as yet not totally demonstrated. CircRNA is one sort of non-coding RNA, and late distributed information proposes that circRNAs assume a significant part in heart diseases; however, their expression profiles in the peripheral blood of patients with MI are not yet totally characterized. Therefore, RNAs from peripheral blood were recruited for high-throughput RNA-seq analysis. A total of 3,862 circRNAs were distinguished to be remarkably different, including 2,738 circRNAs being upregulated and 1,124 circRNAs being downregulated. circTMEM165, circUBAC2, circZNF609, circANKRD12, and circSLC8A1 were reconfirmed by RT-qPCR in the cell model. ROC curves uncovered that they have great sensitivity and specificity in the determination of MI. Besides, circRNAs are associated with cell metabolism and function by directing complex networks among circular RNAs, microRNAs, and messenger RNAs. In outline, our study portrayed the specific articulation profiles of circular RNA in patients with MI. The outcomes showed that circRNAs might fill in as a sort of ideal biomarkers for MI diagnosis. Further exploration of these circRNAs may enrich our understanding of MI etiology and progression.

【 授权许可】

CC BY