Ulcerative Colitis: Novel Epithelial Insights Provided by Single Cell RNA Sequencing

Frontiers in Medicine

article

Joao M. Serigado¹  Jennifer Foulke-Abel²  William C. Hines³  Joshua A Hanson⁴  Julie In¹  Olga Kovbasnjuk¹ 

[1] Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of New Mexico Health Sciences Center;Division of Gastroenterology and Hepatology, Department of Medicine, Johns Hopkins University School of Medicine;Department of Biochemistry and Molecular Biology, University of New Mexico Health Sciences Center;Department of Pathology, University of New Mexico Health Sciences Center;Department of Microbiology and Immunology, University of Maryland School of Medicine

关键词: Ulcerative Colitis;    single cell RNA sequencing;    intestinal epithelium;    goblet cells;    colonic microfold cells;    stem cells;   

DOI  :  10.3389/fmed.2022.868508

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Ulcerative Colitis (UC) is a chronic inflammatory disease of the intestinal tract for which a definitive etiology is yet unknown. Both genetic and environmental factors have been implicated in the development of UC. Recently, single cell RNA sequencing (scRNA-seq) technology revealed cell subpopulations contributing to the pathogenesis of UC and brought new insight into the pathways that connect genome to pathology. This review describes key scRNA-seq findings in two major studies by Broad Institute and University of Oxford, investigating the transcriptomic landscape of epithelial cells in UC. We focus on five major findings: (1) the identification of BEST4 + cells, (2) colonic microfold (M) cells, (3) detailed comparison of the transcriptomes of goblet cells, and (4) colonocytes and (5) stem cells in health and disease. In analyzing the two studies, we identify the commonalities and differences in methodologies, results, and conclusions, offering possible explanations, and validated several cell cluster markers. In systematizing the results, we hope to offer a framework that the broad scientific GI community and GI clinicians can use to replicate or corroborate the extensive new findings that RNA-seq offers.

【 授权许可】

CC BY