From Macroscopy to Ultrastructure: An Integrative Approach to Pulmonary Pathology

Frontiers in Medicine

article

Stijn E. Verleden¹  Peter Braubach⁴  Christopher Werlein⁵  Edith Plucinski⁴  Mark P. Kuhnel⁴  Annemiek Snoeckx⁶  Haroun El Addouli⁶  Tobias Welte⁴  Axel Haverich⁴  Florian P. Laenger⁴  Sabine Dettmer⁴  Patrick Pauwels^1,10  Veronique Verplancke²  Paul E. Van Schil¹  Therese Lapperre²  Johanna M. Kwakkel-Van-Erp²  Maximilian Ackermann^1,12  Jeroen M. H. Hendriks¹  Danny Jonigk⁴ 

[1] Antwerp Surgical Training, Anatomy and Research Centre ,(ASTARC), Antwerp University;Division of Pneumology, University Hospital Antwerp;Department of Thoracic and Vascular Surgery, University Hospital Antwerp;Member of the German Center for Lung Research ,(DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover;Institute for Pathology, Hannover Medical School;Division of Radiology, University Hospital Antwerp and University of Antwerp;Division of Pneumology, Hannover Medical School;Division of Thoracic Surgery, Hannover Medical School;Department of Radiology, Hannover Medical School;Division of Pathology, University Hospital Antwerp;Laboratory of Experimental Medicine and Pediatrics ,(LEMP), Antwerp University;Institute of Pathology and Department of Molecular Pathology, Helios University Clinic Wuppertal, University of Witten-Herdecke;Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University Mainz

关键词: microCT;    lung;    histology;    lung disease;    imaging;   

DOI  :  10.3389/fmed.2022.859337

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Pathology and radiology are complimentary tools, and their joint application is often crucial in obtaining an accurate diagnosis in non-neoplastic pulmonary diseases. However, both come with significant limitations of their own: Computed Tomography (CT) can only visualize larger structures due to its inherent–relatively–poor resolution, while (histo) pathology is often limited due to small sample size and sampling error and only allows for a 2D investigation. An innovative approach of inflating whole lung specimens and subjecting these subsequently to CT and whole lung microCT allows for an accurate matching of CT-imaging and histopathology data of exactly the same areas. Systematic application of this approach allows for a more targeted assessment of localized disease extent and more specifically can be used to investigate early mechanisms of lung diseases on a morphological and molecular level. Therefore, this technique is suitable to selectively investigate changes in the large and small airways, as well as the pulmonary arteries, veins and capillaries in relation to the disease extent in the same lung specimen. In this perspective we provide an overview of the different strategies that are currently being used, as well as how this growing field could further evolve.

【 授权许可】

CC BY