Metagenomics Approaches to Investigate the Neonatal Gut Microbiome

Frontiers in Pediatrics

article

Zakia Boudar¹  Sofia Sehli¹  Sara El Janahi¹  Najib Al Idrissi²  Salsabil Hamdi³  Nouzha Dini⁴  Hassan Brim⁵  Saaïd Amzazi⁶  Chakib Nejjari⁷  Michele Lloyd-Puryear⁹  Hassan Ghazal¹ 

[1] Department of Fundamental Sciences, School of Medicine, Mohammed VI University of Health Sciences;Department of Surgery, Faculty of Medicine, Mohammed VI University of Health Sciences;Laboratory of Genomics and Bioinformatics, School of Pharmacy, Mohammed VI University of Health Sciences;Mother and Child Department, Cheikh Khalifa International University Hospital, Mohammed VI University of Health Sciences;Department of Pathology, Howard University;Laboratory of Human Pathologies Biology, Department of Biology, Faculty of Sciences, and Genomic Center of Human Pathologies, Faculty of Medicine and Pharmacy, Mohammed V University;Department of Epidemiology and Biostatistics, International School of Public Health, Mohammed VI University of Health Sciences;Department of Epidemiology and Public Health, Faculty of Medicine, University Sidi Mohammed Ben Abdellah;American College of Medical Genetics and Genomics;National Center for Scientific and Technical Research

关键词: neonatal microbiota;    microbiome;    metagenomics;    dysbiosis;    colonization;    womb sterile;    delivery;    breastfeeding;   

DOI  :  10.3389/fped.2022.886627

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Early infancy is critical for the development of an infant's gut flora. Many factors can influence microbiota development during the pre- and postnatal periods, including maternal factors, antibiotic exposure, mode of delivery, dietary patterns, and feeding type. Therefore, investigating the connection between these variables and host and microbiome interactions in neonatal development would be of great interest. As the “unculturable” era of microbiome research gives way to an intrinsically multidisciplinary field, microbiome research has reaped the advantages of technological advancements in next-generation sequencing, particularly 16S rRNA gene amplicon and shotgun sequencing, which have considerably expanded our knowledge about gut microbiota development during early life. Using omics approaches to explore the neonatal microbiome may help to better understand the link between the microbiome and newborn diseases. Herein, we summarized the metagenomics methods and tools used to advance knowledge on the neonatal microbiome origin and evolution and how the microbiome shapes early and late individuals' lives for health and disease. The way to overcome limitations in neonatal microbiome studies will be discussed.

【 授权许可】

CC BY