Metagenomics Approaches to Investigate the Neonatal Gut Microbiome

Frontiers in Pediatrics

Hassan Ghazal¹  Michele Lloyd-Puryear²  Chakib Nejjari⁴  Sofia Sehli⁵  Sara El Janahi⁵  Zakia Boudar⁵  Hassan Brim⁶  Najib Al Idrissi⁷  Salsabil Hamdi⁸  Saaïd Amzazi⁹  Nouzha Dini^1,10 

[1] 0National Center for Scientific and Technical Research, Rabat, Morocco;American College of Medical Genetics and Genomics, Bethesda, MD, United States;Department of Epidemiology and Biostatistics, International School of Public Health, Mohammed VI University of Health Sciences, Casablanca, Morocco;Department of Epidemiology and Public Health, Faculty of Medicine, University Sidi Mohammed Ben Abdellah, Fez, Morocco;Department of Fundamental Sciences, School of Medicine, Mohammed VI University of Health Sciences, Casablanca, Morocco;Department of Pathology, Howard University, Washington, DC, United States;Department of Surgery, Faculty of Medicine, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco;Laboratory of Genomics and Bioinformatics, School of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco;Laboratory of Human Pathologies Biology, Department of Biology, Faculty of Sciences, and Genomic Center of Human Pathologies, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco;Mother and Child Department, Cheikh Khalifa International University Hospital, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco;

关键词: neonatal microbiota;    microbiome;    metagenomics;    dysbiosis;    colonization;    womb sterile;   

DOI  :  10.3389/fped.2022.886627

来源: DOAJ

【 摘 要 】

Early infancy is critical for the development of an infant's gut flora. Many factors can influence microbiota development during the pre- and postnatal periods, including maternal factors, antibiotic exposure, mode of delivery, dietary patterns, and feeding type. Therefore, investigating the connection between these variables and host and microbiome interactions in neonatal development would be of great interest. As the “unculturable” era of microbiome research gives way to an intrinsically multidisciplinary field, microbiome research has reaped the advantages of technological advancements in next-generation sequencing, particularly 16S rRNA gene amplicon and shotgun sequencing, which have considerably expanded our knowledge about gut microbiota development during early life. Using omics approaches to explore the neonatal microbiome may help to better understand the link between the microbiome and newborn diseases. Herein, we summarized the metagenomics methods and tools used to advance knowledge on the neonatal microbiome origin and evolution and how the microbiome shapes early and late individuals' lives for health and disease. The way to overcome limitations in neonatal microbiome studies will be discussed.

【 授权许可】

Unknown