Viral Metagenomics Reveals Widely Diverse Viral Community of Freshwater Amazonian Lake

Frontiers in Public Health

article

Wylerson Guimarães Nogueira¹  Bruna Verônica Azevedo Gois²  Kenny da Costa Pinheiro²  Andressa de Oliveira Aragão³  Ana Lídia Cavalcante Queiroz²  Artur Luiz da Silva³  Adriana Carneiro Folador³  Rommel Thiago Jucá Ramos² 

[1] Department of Biochemistry and Immunology, Federal University of Minas Gerais;Institute of Biological Sciences, Federal University of Pará;Laboratory of Genomic and Bioinformatics, Center of Genomics and System Biology, Federal University of Pará

关键词: Amazon;    Lake Bolonha;    freshwater;    viruses;    bacteriophages;    cyanophages;    virome;    metagenomics;   

DOI  :  10.3389/fpubh.2022.869886

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Despite the importance of understanding the ecology of freshwater viruses, there are not many studies on the subject compared to marine viruses. The microbiological interactions in these environments are still poorly known, especially between bacteriophages and their host bacteria and between cyanophages and cyanobacteria. Lake Bologna, Belém, capital of the Brazilian State of Pará, is a water source that supplies the city and its metropolitan region. However, it remains unexplored regarding the contents of its virome and viral diversity composition. Therefore, this work aims to explore the taxonomic diversity of DNA viruses in this lake, especially bacteriophages and cyanophages, since they can act as transducers of resistance genes and reporters of water quality for human consumption. We used metagenomic sequencing data generated by previous studies. We analyzed it at the taxonomic level using the tools Kraken2, Bracken, and Pavian; later, the data was assembled using Genome Detective, which performs the assembly of viruses. The results observed here suggest the existence of a widely diverse viral community and established microbial phage-regulated dynamics in Lake Bolonha. This work is the first ever to describe the virome of Lake Bolonha using a metagenomic approach based on high-throughput sequencing, as it contributes to the understanding of water-related public health concerns regarding the spreading of antibiotic resistance genes and population control of native bacteria and cyanobacteria.

【 授权许可】

CC BY