The Impact of Pre-analytical Quality Initiatives on Cholangiocarcinoma Diagnostics in Thailand

Frontiers in Public Health

article

Supinda Koonmee¹  Sakkarn Sangkhamanon¹  Piyapharom Intarawichian¹  Chaiwat Aphivatanasiri¹  Waritta Kunprom¹  Prakasit Sa-ngiamwibool¹  Suwit Balthaisong¹  Chitsakul Phuyao¹  Piya Prajumwongs¹  Reza Alaghehbandan⁴  Malinee Thanee¹ 

[1] Cholangiocarcinoma Screening and Care Program, Khon Kaen University;Cholangiocarcinoma Research Institute, Khon Kaen University;Department of Pathology, Faculty of Medicine, Khon Kaen University;Department of Pathology, Faculty of Medicine, Royal Columbian Hospital, University of British Columbia

关键词: cholangiocarcinoma;    pre-analytical phase;    tissue preservation;    immunohistochemistry;    histopathology;   

DOI  :  10.3389/fpubh.2022.792847

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Cholangiocarcinoma (CCA) is the most prevalent malignancy in Thailand, with unfortunate late diagnosis and frequent metastatic disease outcomes. An accurate tissue diagnosis is the first and most important step in the treatment of CCA. Tissue quality and preservation during the pre-analytical phase play major roles in the proper histological evaluation and potential biomarker testing. This study evaluated the impact of using the “Cholangiocarcinoma Screening and Care Program (CASCAP)” container, as an innovative tool to address pre-analytical challenges faced by pathology laboratories in Thailand. This is a comparison study examining the quality of CCA specimens using the CASCAP container vs. the conventional method, using hematoxylin and eosin (H&E) and immunohistochemistry (IHC). CCA tissue quality using the CASCAP container significantly reduced artifact deposition while improving the cellular structure and nuclear and cytoplasmic morphologies. The immunohistochemical expression of cytokeratin 19 (CK19), a prognostic marker in CCA, significantly improved in the CASCAP container group in comparison with the conventional method. This innovation is proven to significantly enhance the CCA tissue quality diagnostics and prognostic biomarker testing, hence improving overall cancer care, diagnosis, and treatment in Thailand.

【 授权许可】

CC BY