C. acnes qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment

Frontiers in Public Health

article

Jingheng Zhang¹  Fang Yu¹  Keyun Fu¹  Xinyu Ma¹  Yi Han¹  Chi Ching Ali¹  Haonan Zhou²  Yantao Xu¹  Tingyue Zhang¹  Shuntong Kang¹  Yiming Xu¹  Zhuolin Li²  Jiaqi Shi²  Shuai Gao²  Yongyi Chen⁴  Liyu Chen⁶  Jianglin Zhang⁷  Feizhou Zhu² 

[1] Xiangya School of Medicine, Central South University;Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University;Department of Dermatology, Xiangya Hospital, Central South University;Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University;Clinical Research Center for Wound Healing in Hunan Province;Department of Microbiology, School of Basic Medical Sciences, Central South University;Department of Dermatology, Shenzhen People's Hospital;The Second Clinical Medical College, Jinan University;The First Affiliated Hospital, Southern University of Science and Technology;Hunan Key Laboratory of Animal Models for Human Diseases

关键词: acne (acne vulgaris);    antimicrobial resistance;    quantitative PCR (qPCR);    antimicrobial;    macrolide-resistant gene;   

DOI  :  10.3389/fpubh.2022.787299

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Background Macrolides have been widely used to treat moderate-to-severe acne for more than 50 years. However, the prevalent antibiotic resistance of Propionibacterium acnes, along with the absence of clinically available resistance tests, has made macrolide misuse a frequent occurrence around the globe, with serious consequences. Objective We developed Cutibacterium acnes quantitative PCR (qPCR)-based antibiotics resistance assay (ACQUIRE) to enable fast and accurate detection of C. acnes macrolide resistance in clinical settings, representing an opportunity to administer antibiotics more wisely and improve the quality of care. Methods A cross-sectional observational study ( n = 915) was conducted to probe into the macrolide resistance of C. acnes in patients with acne. Results The high sensitivity of ACQUIRE enabled us to reveal a much higher C. acnes 23S recombinant DNA (rDNA) point mutation rate (52%) and thus a higher macrolide resistance (75.5%) compared to previous reports. Carriage of ermX gene was discovered on 472 (53%) subjects, which concurs with previous studies. Conclusion The macrolide resistance of C. acnes is much higher than previously reported. Integrating ACQUIRE into acne treatment modalities may eliminate macrolide misuse and achieve better clinical improvements.

【 授权许可】

CC BY