Ozonized Water in Microbial Control: Analysis of the Stability, In Vitro Biocidal Potential, and Cytotoxicity

Biology

Carlos Nascimento¹  Marcelo Gerhardt¹  Gabriela Monteiro Lobato¹  Arlene Souza Lessa²  Paulo Roberto Freitas Neves³  Eduardo Santos da Silva⁴  Luis Alberto Breda Mascarenhas⁴  Laerte Marlon Conceição dos Santos⁴  Cássio Santana Meira⁴  Leticia de Alencar Pereira Rodrigues⁴  Bruna Aparecida Souza Machado⁴  Fabricia Oliveira Oliveira⁴  Greta Almeida Fernandes Moreira⁴ 

[1] China Three Gorges Corporation—CTG Brazil, Rio Paraná Energia S.A. Rodovia MS-444 s/nº km 58, Ilha Solteira 79590-000, Selviria, Brazil;Gonçalo Moniz Institute, FIOCRUZ Microscopy Service, Technological Platforms Network, Salvador 40296-710, Bahia, Brazil;University Center SENAI/CIMATEC, SENAI Computational Modeling and Industrial Technology, Salvador 41650-010, Bahia, Brazil;University Center SENAI/CIMATEC, SENAI Institute of Innovation in Health Advanced Systems (ISI SAS), Salvador 41650-010, Bahia, Brazil;

关键词: aqueous ozone;    antimicrobial;    antimicrobial resistance;    sanitizer;    cytotoxicity;   

DOI  :  10.3390/biology10060525

来源: DOAJ

【 摘 要 】

O₃ dissolved in water (or ozonized water) has been considered a potent antimicrobial agent, and this study aimed to test this through microbiological and in vitro assays. The stability of O₃ was accessed following modifications of the physicochemical parameters of water, such as the temperature and pH, with or without buffering. Three concentrations of O₃ (0.4, 0.6, and 0.8 ppm) dissolved in water were tested against different microorganisms, and an analysis of the cytotoxic effects was also conducted using the human ear fibroblast cell line (Hfib). Under the physicochemical conditions of 4 °C and pH 5, O₃ remained the most stable and concentrated compared to pH 7 and water at 25 °C. Exposure to ozonized water resulted in high mortality rates for Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans. Scanning electron micrograph images indicate that the effects on osmotic stability due to cell wall lysis might be one of the killing mechanisms of ozonized water. The biocidal agent was biocompatible and presented no cytotoxic effect against Hfib cells. Therefore, due to its cytocompatibility and biocidal action, ozonized water can be considered a viable alternative for microbial control, being possible, for example, its use in disinfection processes.

【 授权许可】

Unknown